Hasni Issam, Armstrong Nicholas, Decloquement Philippe, Azza Said, Fontanini Anthony, Abbe Olivier, Cherif Louazani Amina, Demanèche Sandrine, Chabrière Eric, Colson Philippe, La Scola Bernard
Aix-Marseille Université UM63, Faculté des Sciences Médicales et Paramédicales secteur Timone, Institut de Recherche Pour le Développement IRD 198, Assistance Publique-Hôpitaux de Marseille (AP-HM), 13385 Marseille, France.
R&D Department, Amoéba, 69680 Chassieu, France.
Microorganisms. 2020 Nov 16;8(11):1791. doi: 10.3390/microorganisms8111791.
C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an industry involved in the treatment of microbiological risk in the water and plant protection sectors, has developed a natural biocide based on the property of to manage the proliferation of the pathogen in cooling towers. In axenic liquid medium, amoebas are usually cultivated in adhesion on culture flask. However, we implemented a liquid culture in suspension using bioreactors in order to produce large quantities of . In order to investigate the culture condition effects on , we conducted a study based on microscopic, proteomics and lipidomics analyzes. According to the culture condition, amoeba exhibited two different phenotypes. The differential proteomics study showed that amoebas seemed to promote the lipid metabolism pathway in suspension culture, whereas we observed an upregulation of the carbohydrate pathway in adherent culture. Furthermore, we observed an over-regulation of proteins related to the cytoskeleton for cells grown in adhesion. Regarding the lipid analysis, suspension and adhesion cell growth showed comparable lipid class compositions. However, the differential lipid analysis revealed differences that confirmed cell phenotype differences observed by microscopy and predicted by proteomics. Overall, this study provides us with a better insight into the biology and molecular processes of in different culture lifestyles.
C2c变形虫是一种自由生活的变形虫，它已证明有能力抑制某些菌株的细胞内增殖，这些菌株是栖息于水生环境中的致病细菌。在水和植物保护领域从事微生物风险处理的Amoeba公司，基于其特性开发了一种天然杀菌剂，用于控制冷却塔中病原体的增殖。在无菌液体培养基中，变形虫通常附着在培养瓶上进行培养。然而，为了大量生产，我们采用生物反应器进行悬浮液体培养。为了研究培养条件对的影响，我们基于显微镜、蛋白质组学和脂质组学分析开展了一项研究。根据培养条件，变形虫呈现出两种不同的表型。差异蛋白质组学研究表明，在悬浮培养中变形虫似乎促进脂质代谢途径，而在贴壁培养中我们观察到碳水化合物途径上调。此外，对于贴壁生长的细胞，我们观察到与细胞骨架相关的蛋白质过度调节。关于脂质分析，悬浮培养和贴壁培养的细胞生长显示出可比的脂质类别组成。然而，差异脂质分析揭示的差异证实了通过显微镜观察到并由蛋白质组学预测的细胞表型差异。总体而言，这项研究让我们对在不同培养方式下的生物学和分子过程有了更深入的了解。
