中国首例李属坏死环斑病毒侵染葡萄的报告。
First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China.
机构信息
College of Agriculture, Nanjing Agriculture University, Nanjing, 210095, People's Republic of China.
College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, People's Republic of China.
出版信息
Virol J. 2020 Nov 18;17(1):181. doi: 10.1186/s12985-020-01438-3.
BACKGROUND
Virus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China.
OBJECTIVE
The leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle.
METHODS
Small RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1 CONCLUSIONS: This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.
背景
病毒病是葡萄藤的主要病害之一，在中国尚未有李皮坏死和茎陷斑相关病毒感染葡萄藤的报道。
目的
从山东省采集了酿酒葡萄品种‘Cabernet Gernischt’的叶片样本，这些叶片出现了斑点和皱缩等类似病毒的症状。
方法
采用小 RNA 测序结合 RT-PCR 方法对田间样本中的潜在病毒进行检测。采用 MEGA 5.1 的邻接法构建系统发育树。
结论
这是中国首例李皮坏死和茎陷斑相关病毒感染葡萄藤的报道，有助于更好地了解该病原体的流行病学和宿主范围分布。
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