Department of Pharmacy, Zhejiang Pharmaceutical College, 888 Yinxian Road, YinZhou District, Ningbo, 315000, Zhejiang, China.
Department of Neurology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Behav Brain Funct. 2020 Nov 18;16(1):10. doi: 10.1186/s12993-020-00172-8.
The purpose of this study was to evaluate the effect of astaxanthin (AST) on cognition function, inflammatory response and oxidative stress in vascular dementia (VD) mice.
VD mice model was established by left unilateral common carotid arteries occlusion (LUCCAO). Following LUCCAO, AST was intragastrically administered for 30 days. Object recognition test and morris water maze test were used to evaluate cognitive function. Hematoxylin and eosin staining was performed to observe the hippocampal neuron structure. Enzyme-linked immunosorbent assay kit and bicinchoninic acid kit were respectively adopted to measure IL-1β and IL-4 protein expression and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hippocampus and prefrontal cortex.
AST improved the discrimination ability of VD mice. The escape latency and path length of VD mice treated with AST were dramatically reduced. Besides, AST 200 mg/kg enhanced crossing platform time and the number of times crossing the platform quadrant, and alleviated the morphological impairment in VD mice. Moreover, we found that AST inhibited IL-1β expression and MDA content, whereas promoted IL-4 expression and SOD activity in a dose-dependent manner.
AST could improve cognitive impairment and hippocampal neurons in VD mice, which may be related to suppression of inflammatory response and oxidative stress.
本研究旨在评估虾青素（AST）对血管性痴呆（VD）小鼠认知功能、炎症反应和氧化应激的影响。
通过左侧颈总动脉闭塞（LUCCAO）建立 VD 小鼠模型。LUCCAO 后，AST 经胃内给药 30 天。采用物体识别试验和 Morris 水迷宫试验评估认知功能。苏木精和伊红染色观察海马神经元结构。酶联免疫吸附试验试剂盒和二辛可宁酸试剂盒分别用于测量海马和前额叶皮质中 IL-1β 和 IL-4 蛋白表达以及超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。
AST 改善了 VD 小鼠的辨别能力。AST 处理的 VD 小鼠的逃避潜伏期和路径长度显著缩短。此外，AST 200mg/kg 增加了穿越平台的时间和穿越平台象限的次数，并减轻了 VD 小鼠的形态损伤。此外，我们发现 AST 呈剂量依赖性抑制 IL-1β 表达和 MDA 含量，而促进 IL-4 表达和 SOD 活性。
AST 可改善 VD 小鼠的认知障碍和海马神经元损伤，这可能与抑制炎症反应和氧化应激有关。
