Institute of Marine Drugs, Guangxi University of Chinese Medicine, Guangxi, China.
Faculty of Pharmacy, Guangxi University of Chinese Medicine, Guangxi, China.
Pharm Biol. 2020 Dec;58(1):1211-1220. doi: 10.1080/13880209.2020.1837187.
Fruit of (Forsk.) Vierh. (Acanthaceae) is used as a Chinese herb. Studies have found that it contains marinoid J, a novel phenylethanoid glycoside (PG) compound, but its neuroprotective functions are largely unknown.
This study evaluated the effects of marinoid J on vascular dementia (VD) and determined its potential mechanisms of action.
The VD model was established by the ligation of the bilateral common carotid artery in Sprague-Dawley rats, who received daily intragastrically administration of saline, marinoid J (125 or 500 mg/kg body weight/d), or oxiracetam (250 mg/kg body weight/d) for 14 days (20 rats in each group). The Morris water maze (MWM) was used to evaluate cognitive performance. The hippocampus was subjected to histological and proteomic analyses.
Marinoid J shortened the escape latency of VD rats (31.07 ± 3.74 s, < 0.05). It also decreased malondialdehyde (MDA) (27.53%) and nitric oxide (NO) (20.41%) while increasing superoxide dismutase (SOD) (11.26%) and glutathione peroxidase (GSH-Px) (20.38%) content in hippocampus tissues. Proteomic analysis revealed 45 differentially expressed proteins (DEPs) in marinoid J-treated VD rats, which included angiotensin-converting enzyme (ACE), keratin 18 (KRT18), cluster of differentiation 34 (CD34), and synaptotagmin II (SYT2).
Marinoid J played a role in protecting hippocampal neurons by regulating a set of proteins that influence oxidative stress and apoptosis, this effect may thereby alleviate the symptoms of VD rats. Thus, pharmacological manipulation of marinoid J may offer a novel opportunity for VD treatment.
（Forsk.） Vierh.（爵床科）的果实被用作中药。研究发现，它含有海洋 J，一种新型苯乙醇苷（PG）化合物，但它的神经保护功能在很大程度上尚不清楚。
本研究评估了海洋 J 对血管性痴呆（VD）的影响，并确定了其潜在的作用机制。
通过结扎 Sprague-Dawley 大鼠双侧颈总动脉建立 VD 模型，大鼠每天给予生理盐水、海洋 J（125 或 500mg/kg 体重/天）或奥拉西坦（250mg/kg 体重/天）灌胃，共 14 天（每组 20 只大鼠）。使用 Morris 水迷宫（MWM）评估认知表现。对海马组织进行组织学和蛋白质组学分析。
海洋 J 缩短了 VD 大鼠的逃避潜伏期（31.07±3.74s，<0.05）。它还降低了丙二醛（MDA）（27.53%）和一氧化氮（NO）（20.41%）的含量，同时增加了超氧化物歧化酶（SOD）（11.26%）和谷胱甘肽过氧化物酶（GSH-Px）（20.38%）在海马组织中的含量。蛋白质组学分析显示，海洋 J 治疗的 VD 大鼠中有 45 种差异表达蛋白（DEPs），包括血管紧张素转换酶（ACE）、角蛋白 18（KRT18）、分化群 34（CD34）和突触结合蛋白 II（SYT2）。
海洋 J 通过调节一组影响氧化应激和细胞凋亡的蛋白质来保护海马神经元，从而缓解 VD 大鼠的症状。因此，对海洋 J 的药理学干预可能为 VD 治疗提供新的机会。
