Liang Guiwen, Wu Ruo, Jiang Lan, Liu Yanfang, Mao Guomin, Huang Zhongwei, Qi Lei, Jiang Haiyan
Department of Emergency Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Department of Gastroenterology, First People's Hospital of Zhangjiagang City, Zhangjiagang, China.
Transl Androl Urol. 2020 Oct;9(5):2192-2199. doi: 10.21037/tau-20-1173.
This experiment aimed to investigate the role and mechanism of lipoprotein-associated phospholipase A2 (Lp-PLA2) in kidney injury in septic mice induced by cecal ligation and perforation (CLP).
Male BALB/c mice were randomly divided into two groups: sham-operation group (Sham group) and septic group (CLP group). The septic model was simulated by cecal ligation and puncture method, but only cecal ligation was used for the sham operation group. The whole serum and renal tissue samples of the mice were collected 24 hours after modeling in both groups. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of renal tissue, the renal injury score was recorded, and the creatinine (Cr) and blood urea nitrogen (BUN) levels were detected by automatic biochemical analyzer, while the serum Lp-PLA2 level was detected by enzyme-linked immunosorbent assay (ELISA). The 7-day survival rate and the survival curve of the two groups were statistically analyzed.
Compared with the Sham group, the pathological score of renal injury in the CLP Group was higher, the level of Lp-PLA2 in serum was significantly increased (all P<0.01), and the expression of Lp-PLA2 in renal tissue was significantly elevated (all P<0.01). Furthermore, the 7-day survival rate of the Sham group was 90%, while that of CLP group was 25%.
The expression level of Lp-PLA2 in blood and kidney tissue of septic mice was increased and correlated with prognosis. However, the predictive value of Lp-PLA2 for prognosis in septic mice needs further study.
本实验旨在探讨脂蛋白相关磷脂酶A2（Lp-PLA2）在盲肠结扎穿孔（CLP）诱导的脓毒症小鼠肾损伤中的作用及机制。
将雄性BALB/c小鼠随机分为两组：假手术组（Sham组）和脓毒症组（CLP组）。采用盲肠结扎穿刺法模拟脓毒症模型，假手术组仅行盲肠结扎。两组建模后24小时采集小鼠全血及肾组织样本。采用苏木精-伊红（HE）染色观察肾组织病理变化，记录肾损伤评分，用自动生化分析仪检测肌酐（Cr）和血尿素氮（BUN）水平，采用酶联免疫吸附测定（ELISA）检测血清Lp-PLA2水平。对两组的7天生存率及生存曲线进行统计学分析。
与Sham组相比，CLP组肾损伤病理评分更高，血清Lp-PLA2水平显著升高（均P<0.01），肾组织中Lp-PLA2表达明显上调（均P<0.01）。此外，Sham组7天生存率为90%，而CLP组为25%。
脓毒症小鼠血液及肾组织中Lp-PLA2表达水平升高且与预后相关。然而，Lp-PLA2对脓毒症小鼠预后的预测价值有待进一步研究。
