Departement of clinical sciences, Faculty of veterinary medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Vet Med Sci. 2021 Mar;7(2):586-592. doi: 10.1002/vms3.398. Epub 2020 Nov 18.
The present prospective randomized experimental study was designed to determine the effects of doxapram on haematological, serum biochemical and antioxidant status in dogs after propofol anaesthesia. Twenty-four healthy male mixed breed dogs, aged 1-2 years, weighing 20.4 ± 2.6 kg was studied. Each dog was anaesthetized twice, with at least one week for washout. Animals were sedated with acepromazine (0.1 mg/kg) intramuscularly. Forty minutes later, anaesthesia was induced using intravenous (IV) propofol (4 mg/kg) titration and maintained for 30 min by propofol (0.2 mg kg min ). After propofol was discontinued, doxapram (2 mg/kg) hydrochloride was administrated IV in PD treatment while an equal volume of saline was administrated in PS treatment. Blood parameters were analysed in four times: immediately before sedation (T1), after treatment (T2), after complete recovery (T3) and 24 hr later (T4). Haematological assessments revealed no significant difference between treatments except in haematocrit which was significantly reduced at T4 (24 hr later) in PD. A decreasing trend of all haematological variables was observed after doxapram administration until recovery, except monocyte, mean corpuscular haemoglobin, red blood cell distribution width and platelet count. Serum urea, creatinine, glucose, cholesterol, direct bilirubin concentration and alanine aminotransferase activity were not changed following doxapram administration compared to the PS treatment. After doxapram administration, Creatinine (T3), Albumin (T2) and Protein (T2 & T3) decreased while Glucose (T2 & T3) and BT (T3) increased. Antioxidant parameters measured showed no difference between treatments or time. Doxapram (2 mg/kg) IV did not induce any major negative effects on haematological, serum biochemical variables and oxidant/antioxidant status in dogs after propofol anaesthesia.
本前瞻性随机实验研究旨在确定多沙普仑对丙泊酚麻醉后犬血液学、血清生化和抗氧化状态的影响。研究对象为 24 只 1-2 岁、体重 20.4±2.6kg 的雄性混合品种犬。每只犬接受两次麻醉，至少间隔一周以清除药物。犬肌肉注射氯丙嗪（0.1mg/kg）镇静，40 分钟后静脉注射（IV）丙泊酚（4mg/kg）滴定诱导麻醉，并用丙泊酚（0.2mg/kg·min）维持 30 分钟。停止丙泊酚后，PD 组给予盐酸多沙普仑（2mg/kg）IV 给药，PS 组给予等容量生理盐水。在四个时间点分析血液参数：镇静前即刻（T1）、治疗后（T2）、完全恢复后（T3）和 24 小时后（T4）。除了 T4（24 小时后）时的红细胞压积显著降低外，两种处理之间的血液学评估没有显著差异。多沙普仑给药后，除单核细胞、平均红细胞血红蛋白、红细胞分布宽度和血小板计数外，所有血液学变量均呈下降趋势，直至恢复。与 PS 治疗相比，多沙普仑给药后血清尿素、肌酐、葡萄糖、胆固醇、直接胆红素浓度和丙氨酸氨基转移酶活性无变化。多沙普仑给药后，肌酐（T3）、白蛋白（T2）和总蛋白（T2 和 T3）降低，而葡萄糖（T2 和 T3）和 BT（T3）增加。抗氧化参数测量显示，两种处理之间或不同时间点均无差异。IV 给予多沙普仑（2mg/kg）对丙泊酚麻醉后犬的血液学、血清生化变量和氧化应激/抗氧化状态没有引起任何重大负面影响。
