酿酒酵母提取物中RNA聚合酶II启动子的准确起始。
Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
作者信息
Lue N F, Kornberg R D
机构信息
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
出版信息
Proc Natl Acad Sci U S A. 1987 Dec;84(24):8839-43. doi: 10.1073/pnas.84.24.8839.
Abstract
A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.
摘要
酵母核提取物支持从CYC1和PYK1启动子进行转录。转录在体外于体内使用的位点处或其附近起始。“TATA”序列的缺失使反应消失。α-鹅膏蕈碱（10微克/毫升）和氯化物（100毫摩尔）具有高度抑制作用。
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