Department of Medical Biotechnology, School of Medical Sciences, Kermanshah University of Medical Science, Kermanshah, Iran.
School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Drug Target. 2021 Apr;29(4):387-402. doi: 10.1080/1061186X.2020.1853756. Epub 2020 Dec 3.
Targeted therapy is one of the favourable methods used in cancer treatment. Several recombinant proteins and small-molecules used for this aim include monoclonal antibodies, antibody fragments and peptides. Nanobody (Nb) is a camelid antibody fragment that is very effective in targeted therapy. Recently, several anti-EGFR (epidermal growth factor receptor) Nbs have been developed and utilised for diagnosis and therapy of EGFR overexpressing tumours. Anti-EGFR Nbs are used in drug delivery systems, photodynamic therapy (PDT) and/or conjugated to other molecules such as quantum dots (QDs), nanoparticles, liposome, tumour penetration peptides, neural stem cells (NSCs) and chimeric antigen receptor T cells (CAR T cells). In this review, we discussed the structure and function of EGFR and Nb, the current status of EGFR targeting, and recent developments in anti-EGFR Nbs. To gain sound insight into the issue at hand, we focused on the most powerful anti-EGFR Nbs.
靶向治疗是癌症治疗中使用的一种有利方法。为此目的而使用的几种重组蛋白和小分子包括单克隆抗体、抗体片段和肽。纳米抗体 (Nb) 是一种非常有效的骆驼科抗体片段，用于靶向治疗。最近，已经开发出了几种针对 EGFR（表皮生长因子受体）的 Nb，并用于诊断和治疗 EGFR 过表达肿瘤。抗 EGFR Nb 用于药物输送系统、光动力疗法 (PDT) 和/或与其他分子如量子点 (QDs)、纳米颗粒、脂质体、肿瘤穿透肽、神经干细胞 (NSCs) 和嵌合抗原受体 T 细胞 (CAR T 细胞) 结合。在这篇综述中，我们讨论了 EGFR 和 Nb 的结构和功能、EGFR 靶向的现状以及抗 EGFR Nb 的最新进展。为了深入了解当前的问题，我们专注于最强大的抗 EGFR Nb。
