Blanton R, Loula E C, Parker J
Department of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9011-4. doi: 10.1073/pnas.84.24.9011.
Infection with Schistosoma mansoni, a parasitic fluke, is acquired when cercariae found in freshwater bodies at ambient temperature penetrate human skin. In this study the response of S. mansoni cercariae to change in temperature from ambient to that of mammalian hosts was compared to the response of other stages that do not experience such dramatic temperature shifts. In cercariae, temperature increases from 23 degrees C to either 37 degrees C or 42 degrees C primarily induced the synthesis of two proteins unique to this stage, of Mr 60,000 and 58,000. Neither protein could be induced by similar treatment in the other two stages of the parasite found in mammalian hosts. Instead, these stages predominantly synthesized a Mr 70,000 protein that crossreacted with monoclonal and polyclonal antibodies against the Mr 70,000 heat shock protein of Drosophila and chicken. No crossreactive material was detected in cercariae, suggesting that a protein homologous to the highly conserved heat shock protein of Mr 70,000 is absent or induced to very low levels by temperature increase at this stage. The induction of the Mr 60,000 and 58,000 proteins was confined to the initial 3-hr period during cercarial transformation to the schistosomula stage.
感染曼氏血吸虫（一种寄生吸虫）是由于在常温淡水中发现的尾蚴穿透人体皮肤所致。在本研究中，将曼氏血吸虫尾蚴对从常温到哺乳动物宿主温度变化的反应，与其他未经历如此剧烈温度变化阶段的反应进行了比较。在尾蚴中，温度从23摄氏度升至37摄氏度或42摄氏度主要诱导了该阶段特有的两种蛋白质的合成，其分子量分别为60,000和58,000。在哺乳动物宿主中发现的寄生虫的其他两个阶段，类似处理均无法诱导这两种蛋白质的产生。相反，这些阶段主要合成了一种分子量为70,000的蛋白质，它能与针对果蝇和鸡的分子量为70,000的热休克蛋白的单克隆和多克隆抗体发生交叉反应。在尾蚴中未检测到交叉反应物质，这表明在该阶段不存在与高度保守的分子量为70,000的热休克蛋白同源的蛋白质，或者温度升高诱导其产生的水平极低。分子量为60,000和58,000的蛋白质的诱导仅限于尾蚴转变为童虫阶段的最初3小时内。
