Yuckenberg P D, Poupin F, Mansour T E
Exp Parasitol. 1987 Jun;63(3):301-11. doi: 10.1016/0014-4894(87)90177-9.
There are alterations in the proteins synthesized during different stages of development of Schistosoma mansoni. The protein profiles at different stages were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When stained by Coomassie blue, no significant differences were seen in protein profiles derived from cultured schistosomula from Days 0 to 6 and from adults. Newly synthesized proteins were detected by [35S]methionine incorporation. There were only a few differences in the protein profiles of schistosomula from Days 1 to 6 and from adults. Profiles derived from Day 0 schistosomula showed striking differences. Only a few proteins appear to be synthesized on Day 0 under these conditions. Schistosomula on Day 0 synthesized several minor proteins as well as a major protein of approximately 69,000 Da. This protein was immunoprecipitated by rabbit antiserum against bovine uncoating adenosine triphosphatase which recognizes the constitutive and induced 70,000 Da heat shock proteins in a wide variety of eukaryotic cells. More significant differences were observed when the newly synthesized proteins were analyzed by two dimensional gel electrophoresis. The profiles of newly synthesized proteins showed a specific repertoire of expression during the early stages of development in the parasite. A shift in temperature and medium during transformation from aquarium water to isotonic medium may initiate the synthesis of heat shock protein in these parasites.
曼氏血吸虫发育的不同阶段所合成的蛋白质存在变化。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析不同阶段的蛋白质谱。用考马斯亮蓝染色时，来自第0至6天培养的童虫和成虫的蛋白质谱未见显著差异。通过掺入[35S]甲硫氨酸检测新合成的蛋白质。第1至6天的童虫和成虫的蛋白质谱只有一些差异。来自第0天童虫的蛋白质谱显示出显著差异。在这些条件下，第0天似乎只合成了少数几种蛋白质。第0天的童虫合成了几种次要蛋白质以及一种约69,000 Da的主要蛋白质。这种蛋白质能被抗牛脱壳三磷酸腺苷酶的兔抗血清免疫沉淀，该抗血清能识别多种真核细胞中的组成型和诱导型70,000 Da热休克蛋白。当通过二维凝胶电泳分析新合成的蛋白质时，观察到了更显著的差异。新合成蛋白质的谱显示出寄生虫发育早期阶段特定的表达模式。从水族箱水转变为等渗培养基的过程中温度和培养基的变化可能会引发这些寄生虫中热休克蛋白的合成。
