College of Chemistry, Green Catalysis Center, Zhengzhou University, Zhengzhou 450001, P. R. China.
Department of Medicinal Chemistry, Center for Natural Products Drug Discovery and Development (CNPD3), College of Pharmacy, University of Florida, 1345 Center Dr, Gainesville, FL 32610, USA.
J Mater Chem B. 2020 Dec 23;8(48):11090-11095. doi: 10.1039/d0tb01766c.
Herein, we have proposed a colorimetric biosensor for detection of acid phosphatase based on human serum albumin (HSA) templated MnO2 nanosheets (HSA-MnO2 NSs). HSA-MnO2 NSs as an efficient biomimetic oxidase could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) to the coloured oxidation product (oxTMB). Acid phosphatase (ACP) could hydrolyze l-ascorbic acid-2-phosphate (AAP) to produce ascorbic acid, and ascorbic acid could lead to the decomposition of MnO2 NSs to Mn2+ ions, inhibiting the production of oxTMB. On the basis of this, we have demonstrated a novel colorimetric approach for the detection of acid phosphatase with the linear range from 50 μU mL-1 to 1500 μU mL-1 and a detection limit of 40 μU mL-1. The MnO2 NS-based colorimetric method has been successfully used to determine the content of acid phosphatase in real samples with satisfactory results.
在此，我们提出了一种基于人血清白蛋白（HSA）模板的 MnO2 纳米片（HSA-MnO2 NSs）用于检测酸性磷酸酶的比色生物传感器。HSA-MnO2 NSs 作为一种有效的模拟酶，可以催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化，生成有色氧化产物（oxTMB）。酸性磷酸酶（ACP）可以水解 l-抗坏血酸-2-磷酸（AAP）生成抗坏血酸，抗坏血酸会导致 MnO2 NSs 分解为 Mn2+离子，从而抑制 oxTMB 的生成。基于这一点，我们提出了一种用于检测酸性磷酸酶的新型比色方法，其线性范围为 50 μU mL-1至 1500 μU mL-1，检测限为 40 μU mL-1。基于 MnO2 NS 的比色法已成功用于测定实际样品中酸性磷酸酶的含量，结果令人满意。
