He Liuying, Wang Feiyang, Chen Ying, Liu Yueying
Department of Chemistry, Capital Normal University, Beijing, People's Republic of China.
Luminescence. 2018 Feb;33(1):145-152. doi: 10.1002/bio.3384. Epub 2017 Aug 30.
In this paper, we report a colorimetric sensor for the rapid, selective detection of ascorbic acid (AA) in aqueous solutions. Single-layered MnO nanosheets were established as an artificial oxidase; consequently colorless 3,3´,5,5´-tetramethylbenzidine (TMB) was oxidized to a blue product (oxTMB), with increase in absorbance at 650 nm. The absorbance of the reaction system decreased after introduction AA, which reduced MnO into Mn . Under optimum conditions, a detection limit of 62.81 nM for AA in aqueous solutions could be achieved. The linear response range for AA was 0.25-30 μM with a correlation coefficient of 0.996. Importantly, the MnO nanosheet-TMB chromogenic reaction exhibited great selectivity as there was no interference from other metal ions, amino acids and small biological molecules. The proposed colorimetric sensing of AA could be applied for fruit, juice and pharmaceutical samples. Moreover, the proposed sensor showed satisfying performance, including low cost, easy preparation, rapid detection, and good biocompatibility.
在本文中，我们报道了一种用于快速、选择性检测水溶液中抗坏血酸（AA）的比色传感器。单层MnO纳米片被用作人工氧化酶；因此，无色的3,3´,5,5´-四甲基联苯胺（TMB）被氧化为蓝色产物（oxTMB），在650 nm处吸光度增加。引入AA后，反应体系的吸光度降低，因为AA将MnO还原为Mn 。在最佳条件下，水溶液中AA的检测限可达62.81 nM。AA的线性响应范围为0.25 - 30 μM，相关系数为0.996。重要的是，MnO纳米片 - TMB显色反应具有很高的选择性，不受其他金属离子、氨基酸和小生物分子的干扰。所提出的AA比色传感可应用于水果、果汁和药物样品。此外，所提出的传感器表现出令人满意的性能，包括低成本、易于制备、快速检测和良好的生物相容性。
