Abramova I M, Bessonov V V, Bogachuk M N, Krivchenko V A, Makarenko M A, Sokurenko M S, Solovyov A O, Turshatov M V, Shevyakova L V
All-Russian Scientific Research Institute of Food Biotechnology - a Branch of the Federal Research Center for Nutrition, Biotechnology and Food Safety, 111033, Moscow, Russian Federation.
Federal Research Center of Nutrition, Biotechnology and Food Safety, 109240, Moscow, Russian Federation.
Vopr Pitan. 2020;89(5):110-118. doi: 10.24411/0042-8833-2020-10071. Epub 2020 Sep 20.
In the process of grain processing for ethyl alcohol, practically only carbohydrates are consumed, which are presented mainly in the form of starch. The remaining components (protein, fats, fiber, minerals) in transit pass into the grain fiber remaining after distillation of the alcohol from the mash. Distillery grain fiber surpasses wheat bran in its indicators, since during the processing it is enriched with biomass of alcohol yeast. In addition, there is a technological possibility of its additional enrichment with protein, amino acids, and vitamins due to changes in the modes of alcoholic fermentation. of the work was to assess the influence of the mode and conditions of alcoholic fermentation on the composition of distillery grain fiber. . Under laboratory conditions, samples of grain fiber of alcohol production were obtained by the method of fermentation samples from wheat under various fermentation conditions. In the obtained samples, the following parameters were determined: protein according to Barnstein, crude protein, vitamins B1, B2, B6, E, as well as the amino acid composition. The volume fraction of ethyl alcohol, the mass concentration of fermentable carbohydrates, and the concentration of yeast cells were determined in intermediate products of alcohol production. . Studies on the effect of yeast from various manufacturers on fermentation rates, biomass growth and grain fiber composition showed the advantage of race Y-717 in terms of alcohol accumulation (11.5% vol.), Fermentation rate (56 hours) and yeast cell concentration (260 million/cm), which was 15-30% more than in other options. However, from the point of view of increasing the content of protein according to Barnstein and crude protein in samples of grain fiber with yeast Y-717, an increase of only 3-4% was noted compared with other options. This was associated with a decrease in the concentration of yeast by 72 h of fermentation due to autolysis. Studies on the influence of the initial yeast concentration on the growth of biomass, the fermentation rate and grain fiber indices showed that with an increase in the initial yeast concentration from 15 to 45 million/cm, the fermentation time reduced to 48 h, the biomass growth at the end of fermentation was 20%, the protein in grain fiber increased by 15%, the content of vitamins B1, B2, B6 and E as well as amino acids increased by 13-17%. . According to the results of the studies, the technological possibility of enriching distillery grain fiber with protein, amino acids, and vitamins due to a change in the fermentation process during the processing of grain raw materials to alcohol is shown. In particular, this could be achieved through the use of yeast with a high growth rate, by increasing the concentration of yeast biomass, shortening the fermentation period and preventing yeast autolysis at the maturation stage.
在用于生产乙醇的谷物加工过程中,实际上只有碳水化合物被消耗,而碳水化合物主要以淀粉的形式存在。其余成分(蛋白质、脂肪、纤维、矿物质)在加工过程中进入从醪液中蒸馏出酒精后剩余的谷物纤维中。酒糟纤维在各项指标上超过麦麸,因为在加工过程中它富集了酒精酵母的生物质。此外,由于酒精发酵方式的改变,在技术上有可能使其进一步富含蛋白质、氨基酸和维生素。本研究的目的是评估酒精发酵方式和条件对酒糟纤维成分的影响。在实验室条件下,通过在不同发酵条件下对小麦进行发酵取样的方法获得酒精生产的谷物纤维样品。在所获得的样品中,测定了以下参数:根据巴恩斯坦法测定的蛋白质、粗蛋白、维生素B1、B2、B6、E以及氨基酸组成。在酒精生产的中间产物中测定了乙醇的体积分数、可发酵碳水化合物的质量浓度和酵母细胞浓度。对来自不同厂家的酵母对发酵速率、生物质生长和谷物纤维成分的影响进行的研究表明,Y - 717菌株在酒精积累(11.5%体积分数)、发酵速率(56小时)和酵母细胞浓度(2.6亿个/立方厘米)方面具有优势,比其他菌株高15 - 30%。然而,从提高使用Y - 717酵母的谷物纤维样品中巴恩斯坦法测定的蛋白质和粗蛋白含量的角度来看,与其他菌株相比仅增加了3 - 4%。这与发酵72小时时酵母浓度因自溶而降低有关。对初始酵母浓度对生物质生长、发酵速率和谷物纤维指标的影响进行的研究表明,随着初始酵母浓度从1500万个/立方厘米增加到4500万个/立方厘米,发酵时间缩短至48小时,发酵结束时生物质生长量为20%,谷物纤维中的蛋白质增加了15%,维生素B1、B2、B6和E以及氨基酸的含量增加了13 - 17%。根据研究结果,表明在将谷物原料加工成酒精的过程中,通过改变发酵过程,在技术上有可能使酒糟纤维富含蛋白质、氨基酸和维生素。具体而言,这可以通过使用生长速率高的酵母、提高酵母生物质浓度、缩短发酵周期以及在成熟阶段防止酵母自溶来实现。