Skotnicki M L, Rolfe B G
Aust J Biol Sci. 1977 Apr;30(1-2):141-53.
Storage in dimethyl sulphoxide (DMSO) of Escherichia coli K12 hybrids carrying nif+ genes from Klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. Similar results are obtained with E. coli K12 hybrids containing the nitrogen-fixing capacity from Rhizobium trifolii. DMSO appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K12 and four chromosomal regions (chlD, chlG, his and unc) are associated with resistance to DMSO.
将携带肺炎克雷伯氏菌固氮基因的大肠杆菌K12杂种储存在二甲基亚砜（DMSO）中会导致选择出有缺陷的固氮表型。含有三叶草根瘤菌固氮能力的大肠杆菌K12杂种也得到了类似的结果。DMSO似乎会影响与大肠杆菌K12能量代谢相关的特定内膜蛋白，并且四个染色体区域（chlD、chlG、his和unc）与对DMSO的抗性有关。
