通过二甲基亚砜对大肠杆菌K-12中固氮缺陷表型进行逐步筛选。
Stepwise selection of defective nitrogen-fixing phenotypes in Escherichia coli K-12 by dimethyl sulfoxide.
作者信息
Skotnicki M L, Rolfe B G
出版信息
J Bacteriol. 1977 May;130(2):939-42. doi: 10.1128/jb.130.2.939-942.1977.
Abstract
Storage in dimethyl sulfoxide of Escherichia coli K-12 hybrids carrying Klebsiella pneumoniae nif+ genes can result in selection of a defective nitrogen-fixing phenotype. Dimethyl sulfoxide appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K-12.
摘要
携带肺炎克雷伯菌固氮基因的大肠杆菌K-12杂种菌在二甲基亚砜中保存会导致选择出有缺陷的固氮表型。二甲基亚砜似乎会影响与大肠杆菌K-12能量代谢相关的特定内膜蛋白。
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Stepwise selection of defective nitrogen-fixing phenotypes in Escherichia coli K-12 by dimethyl sulfoxide.通过二甲基亚砜对大肠杆菌K-12中固氮缺陷表型进行逐步筛选。
J Bacteriol. 1977 May;130(2):939-42. doi: 10.1128/jb.130.2.939-942.1977.
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