Din Hazirah Mohd, Rashed Osamah, Ahmad Khairulmazmi
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Institute of Plantation Studies, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Trop Life Sci Res. 2020 Oct;31(3):29-45. doi: 10.21315/tlsr2020.31.3.3. Epub 2020 Oct 15.
wilt disease is one of the most problematic and destructive disease in cucumber production. The causative agents are and . These pathogens are soil borne and transmitted through infested soil and water. A field survey was conducted to study the disease prevalence in the major growing areas of cucumber in Peninsular Malaysia. Field study revealed that the disease was highly prevalence in the field with the disease incidence was in the range of 10%-60%. The morphological properties of . are microconidia (3.8-15.7 μm × 2.9-4.9 μm), macroconidia (14.8-38.5 μm × 2.4-5.7 μm) and number of septate was 1-4. While for are microconidia (3.39-14.63 μm × 2.36-4.44 μm), macroconidia (7.22-50.46 μm × 2.43-6.14 μm) and number of septate was 1-5. Based on molecular identification had confirmed that the disease is caused by and with similarity index of 99%-100% based on internal transcribed spacer (ITS) gene sequences. The pathogenicity test showed that the symptoms of wilt disease was firstly appeared as yellowing of old leaves. Progressively, the infected plant will be wilted and finally died. The outputs of this study are highly important to establish an effective disease management programme to reduce disease prevalence and yield loss in the field.
枯萎病是黄瓜生产中最具问题性和破坏性的病害之一。病原体是[此处原文缺失病原体名称]和[此处原文缺失病原体名称]。这些病原体是土壤传播的，通过受侵染的土壤和水传播。在马来西亚半岛黄瓜的主要种植区进行了田间调查，以研究该病的流行情况。田间研究表明，该病在田间高度流行，发病率在10%-60%之间。[此处原文缺失病原体名称]的形态特征为：小型分生孢子（3.8-15.7μm×2.9-4.9μm），大型分生孢子（14.8-38.5μm×2.4-5.7μm），分隔数为1-4个。而[此处原文缺失病原体名称]的形态特征为：小型分生孢子（3.39-14.63μm×2.36-4.44μm），大型分生孢子（7.22-50.46μm×2.43-6.14μm），分隔数为1-5个。基于分子鉴定已确认该病由[此处原文缺失病原体名称]和[此处原文缺失病原体名称]引起，基于内转录间隔区（ITS）基因序列的相似性指数为99%-100%。致病性试验表明，[此处原文缺失病原体名称]枯萎病的症状首先表现为老叶变黄。逐渐地，受感染的植株会枯萎并最终死亡。本研究的结果对于制定有效的病害管理计划以降低田间病害流行率和产量损失非常重要。
