Azman Najihah, Zainudin Nur Ain Izzati Mohd, Ibrahim Wan Norhamidah Wan
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Trop Life Sci Res. 2020 Oct;31(3):91-107. doi: 10.21315/tlsr2020.31.3.7. Epub 2020 Oct 15.
Fumonisin B (FB) is a common mycotoxin produced by species particularly and . The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB based on the amplification of gene, to quantify FB produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB and to determine EC toward the larvae of zebrafish (). Fifty isolates of species were isolated from different hosts throughout Malaysia. Successful amplification of the gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB produced by isolate B2433 was 6677.32 ppm meanwhile isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB on larvae of zebrafish, five concentrations of FB (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of in every isolate can be detected by gene analysis and both of the species produced different concentrations of FB. This is the first report of FB produced by species gave a significant effect on zebrafish development.
伏马菌素B（FB）是由 种特别是 和 种产生的一种常见霉菌毒素。产生的毒素会对人类和动物产生不良影响。本研究的目的是基于 基因的扩增检测FB的产生，使用超快速液相色谱（UFLC）分析对分离株产生的FB进行定量，检测FB的胚胎毒性作用，并确定对斑马鱼（ ）幼体的半数效应浓度（EC）。从马来西亚各地不同宿主中分离出50株 种分离株。 基因的成功扩增表明50株分离株中有48株的基因组中存在该基因（800 bp）。分离株B2433产生的FB最高水平为6677.32 ppm，而分离株J1363为954.01 ppm。通过对FB对斑马鱼幼体的胚胎毒性试验评估，测试了五种FB浓度（0.43 ppm、0.58 ppm、0.72 ppm、0.87 ppm和1.00 ppm）。在受精后24至168小时观察暴露于FB的幼体的形态变化。在暴露144小时时，斑马鱼幼体的死亡率和异常率显著增加。同时，自发尾卷曲显示出显著差异。心率没有显著差异。总之，通过 基因分析可以检测到每个分离株中 的存在，并且这两种 种产生的FB浓度不同。这是关于 种产生的FB对斑马鱼发育有显著影响的首次报道。
