Fumonisin B Production by Species and Mycotoxigenic Effect on Larval Zebrafish.

Fumonisin B (FB) is a common mycotoxin produced by species particularly and . The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB based on the amplification of gene, to quantify FB produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB and to determine EC toward the larvae of zebrafish (). Fifty isolates of species were isolated from different hosts throughout Malaysia. Successful amplification of the gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB produced by isolate B2433 was 6677.32 ppm meanwhile isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB on larvae of zebrafish, five concentrations of FB (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of in every isolate can be detected by gene analysis and both of the species produced different concentrations of FB. This is the first report of FB produced by species gave a significant effect on zebrafish development.