College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):2619-2621. doi: 10.1080/22221751.2020.1854057.
oocysts in the environment are a threat to humans and animals. This study is aimed to evaluate the prevalence of in white spoonbills and isolate viable from white spoonbills. In 28.6% (2/7) of white spoonbills, antibodies were found in heart juice by the modified agglutination test (cut-off: 1:4). DNA was detected in tissues of 42.9% (3/7) white spoonbills. One viable strain, named TgSpoonbillCHn1, was isolated from the myocardium of a white spoonbill by bioassay in mice. DNA extracted from TgSpoonbillCHn1 tachyzoites was characterized by PCR-restriction fragment length polymorphism with ten markers and the virulence genes ROP5 and ROP18. The results revealed that it was ToxoDB#2 (Type III). The ROP18/ROP5 genotype combination predicts that this strain is avirulent for mice, which is supported by the infection experiments in mice. This is the first report of the isolation of viable strain from white spoonbil. The prevalence of in white spoonbills may be indicative of environmental contamination of oocysts. This report provides direct evidence of white spoonbill as an intermediate host of .
在环境中的卵囊对人类和动物构成威胁。本研究旨在评估白琵鹭中 的流行情况，并从白琵鹭中分离出有活力的 。在 28.6%（2/7）的白琵鹭中，通过改良凝集试验（截止值：1:4）在心液中发现了 抗体。在 42.9%（3/7）的白琵鹭组织中检测到 DNA。从一只白琵鹭的心肌中通过小鼠生物测定法分离到一株名为 TgSpoonbillCHn1 的有活力的 株。从 TgSpoonbillCHn1 速殖子中提取的 DNA 通过 10 个标记物和毒力基因 ROP5 和 ROP18 的 PCR-限制性片段长度多态性进行了特征分析。结果表明它是 ToxoDB#2（III 型）。ROP18/ROP5 基因型组合表明该菌株对小鼠无毒力，这一结果得到了小鼠感染实验的支持。这是首次从白琵鹭中分离出有活力的 株的报道。白琵鹭中 的流行率可能表明卵囊对环境的污染。本报告提供了白琵鹭作为 的中间宿主的直接证据。
