Veterinary Pathology, Henan Agricultural University, Zhengzhou 450000, China.
Zhengzhou Zoo, Zhengzhou 450000, China.
Genes (Basel). 2023 Aug 10;14(8):1606. doi: 10.3390/genes14081606.
Many cases of infection have been reported worldwide in non-human primates (NHPs), especially in captive New World monkeys. However, few studies on toxoplasmosis in Old World monkeys have been conducted. In this study, serological and molecular biological analyses were carried out to look for antibodies and infection in 13 NHPs from China. infection was confirmed in 8 NHP cases. antibodies were detected in 1/5 New World monkeys and in 4/7 Old World monkeys. DNA was detected in 3/5 New World monkeys and 5/7 Old World monkeys. The one ring-tailed lemur was negative for both antibodies and DNA of . The most common clinical manifestations of infection were malaise, poor appetite, emaciation, and foamy nasal discharge. The most common histopathological findings were interstitial pneumonia, necrotic hepatitis, necrotizing myocarditis, lymphadenitis, and necrotic splenitis. One viable strain was successfully isolated from the myocardium of a patas monkey () by bioassay in mice. tachyzoites were obtained from cell cultures and were designated as TgMonkeyCHn2. The genotype of this strain belongs to ToxoDB genotype #9, and the allele of ROP18/ROP5 gene was 3/6. TgMonkeyCHn2 tachyzoites were avirulent in Swiss mice. To our knowledge, this is the first report of fatal toxoplasmosis in a patas monkey. infection in patas monkeys may indicate environmental contamination by oocysts. The patas monkey is a new host record for .
本研究对来自中国的 13 只非人灵长类动物（NHPs）进行了血清学和分子生物学分析，以调查其弓形虫抗体和感染情况。在 8 例 NHP 中确认了 感染。在 5 只新世界猴和 7 只旧世界猴中检测到了 抗体。在 5 只新世界猴和 7 只旧世界猴中检测到了 DNA。1 只环尾狐猴的 抗体和 DNA 均为阴性。感染的最常见临床表现为不适、食欲不振、消瘦和泡沫状鼻分泌物。最常见的组织病理学发现是间质性肺炎、坏死性肝炎、坏死性心肌炎、淋巴结炎和坏死性脾炎。从一只豹纹猴的心肌中通过小鼠生物测定成功分离到一株活的 株（）。从细胞培养物中获得 速殖子，并将其命名为 TgMonkeyCHn2。该菌株的基因型属于 ToxoDB 基因型 #9，ROP18/ROP5 基因的等位基因是 3/6。TgMonkeyCHn2 速殖子在瑞士小鼠中无致病性。据我们所知，这是首例豹纹猴致命性弓形虫病的报告。豹纹猴的 感染可能表明卵囊对环境的污染。豹纹猴是 的新宿主记录。
