Department of Life Science, Kindai University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.
Department of Life Science, Kindai University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.
Neurosci Lett. 2021 Jan 10;741:135506. doi: 10.1016/j.neulet.2020.135506. Epub 2020 Nov 19.
Lysophosphatidic acid receptor 1 (LPA) is a receptor of lysophosphatidic acid (LPA). The present study investigated Lpar mRNA expression in the mouse pituitary gland by RT-PCR, in situ hybridization, and immunohistochemistry. Lpar mRNA was abundantly expressed in the pituitary gland. In situ hybridization and immunohistochemistry revealed over 90 % of a common glycoprotein α-subunit, luteinizing hormone β-subunit, and thyroid-stimulating hormone β-subunit immunoreactive cells co-expressed Lpar mRNA in the anterior pituitary gland, but few growth hormone, adrenocorticotropic hormone, and prolactin cells co-expressed Lpar. Furthermore, Lpar mRNA levels in the pituitary gland were increased after ovariectomy and decreased after E2 administration. These results demonstrate that LPA-mediated signaling may play physiological roles in gonadotropes and thyrotropes in the mouse pituitary gland.
溶血磷脂酸受体 1（LPA1）是溶血磷脂酸（LPA）的受体。本研究通过 RT-PCR、原位杂交和免疫组织化学方法检测了小鼠垂体中 Lpar mRNA 的表达。Lpar mRNA 在垂体中大量表达。原位杂交和免疫组织化学显示，超过 90%的促性腺激素β亚基和促甲状腺激素β亚基共同表达的常见糖蛋白α亚基免疫反应性细胞在前垂体中共同表达 Lpar mRNA，但生长激素、促肾上腺皮质激素和催乳素细胞很少共同表达 Lpar。此外，卵巢切除后小鼠垂体中 Lpar mRNA 水平升高，E2 给药后降低。这些结果表明，LPA 介导的信号转导可能在小鼠垂体中的促性腺激素细胞和促甲状腺激素细胞中发挥生理作用。
