Schellenberger V, Schellenberger U, Mitin Y V, Jakubke H D
Sekton Biowissenschaften der Karl-Marx-Universität Leipzig, German Democratic Republic.
Anal Biochem. 1987 Sep;165(2):327-30. doi: 10.1016/0003-2697(87)90276-4.
An apparatus that allows continuous analysis of protease-catalyzed acyl transfer reactions is described. Hydrolysis reaction is assayed using automatic titration. A continuous determination of amino group concentration by reaction with o-phthalaldehyde gives the rate of peptide bond formation. The apparatus allows the determination of the partition constant for the nucleophile at various nucleophile concentrations from one run.
描述了一种能够连续分析蛋白酶催化的酰基转移反应的装置。水解反应通过自动滴定进行测定。通过与邻苯二甲醛反应对氨基浓度进行连续测定，可得出肽键形成的速率。该装置能够在一次实验中测定不同亲核试剂浓度下亲核试剂的分配常数。
