Zhao Danyang, Zhang Yao, Ji Shaowei, Lu Yao, Bai Xinyu, Yin Mengting, Huang Chusen, Jia Nengqin
The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, PR China.
The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, PR China.
Biosens Bioelectron. 2021 Feb 1;173:112816. doi: 10.1016/j.bios.2020.112816. Epub 2020 Nov 17.
A highly sensitive quenching molecular imprinting (MIP) photoelectrochemical (PEC) sensor was proposed to detect acrylamide (AM) by using the photoactive composite of ZnO and polypyrrole (PPy) as the PEC signal probe. ZnO, with high electron mobility, excellent chemical and thermal stability as well as good biocompatibility, was selected as the photoelectrically active material. A polypyrrole film was formed on the nanodisk ZnO by electrochemical polymerization, and the recognition site of AM was left on the surface of the PPy film by elution, enabling the specific detection of AM. The transfer of electrons will be hindered when AM is adsorbed on the ZnO/PPy nanocomposites surface, which results in the decrease of photocurrent signal. The proposed molecularly imprinted PEC sensor exhibits significant detection performance of AM in the range of 10 M-2.5 × 10 M with a LOD of 2.147 × 10 M (S/N = 3). The use of photoelectrochemical technology combined with molecular imprinting technology enables the PEC sensor to have excellent selectivity, superior repeatability, preferable stability, low cost, and easy construction, providing a new method for the detection of AM. The high recovery rate in the detection of real samples of potato chips and biscuits indicates that the proposed PEC sensor has potential in monitoring the emerging food safety risks.
提出了一种高灵敏度的猝灭型分子印迹（MIP）光电化学（PEC）传感器，以氧化锌（ZnO）和聚吡咯（PPy）的光活性复合材料作为PEC信号探针来检测丙烯酰胺（AM）。ZnO具有高电子迁移率、优异的化学和热稳定性以及良好的生物相容性，被选为光电活性材料。通过电化学聚合在纳米盘状ZnO上形成聚吡咯膜，并通过洗脱在PPy膜表面留下AM的识别位点，从而实现对AM的特异性检测。当AM吸附在ZnO/PPy纳米复合材料表面时，电子转移会受到阻碍，导致光电流信号降低。所提出的分子印迹PEC传感器在10⁻⁶ M - 2.5×10⁻¹¹ M范围内对AM表现出显著的检测性能，检测限为2.147×10⁻¹¹ M（S/N = 3）。光电化学技术与分子印迹技术的结合使PEC传感器具有优异的选择性、卓越的重复性、良好的稳定性、低成本以及易于构建的特点，为AM的检测提供了一种新方法。在薯片和饼干实际样品检测中的高回收率表明，所提出的PEC传感器在监测新出现的食品安全风险方面具有潜力。
