Department of Clinical Studies, New Bolton Center, University of Pennsylvania, Kennett Square, PA, USA.
Equine Vet J. 2021 Sep;53(5):944-954. doi: 10.1111/evj.13392. Epub 2020 Dec 8.
Standing cone beam computed tomography (CT) provides cross-sectional imaging of the caudal cervical articular process joints (CAPJs) in the sedated horse, though the clinical implications of osteoarthritis (OA) identified on CT in this location are unknown. Increases in concentrations of intra-synovial cytokines could lend support to the clinical significance of CAPJ OA identified on this imaging modality.
Investigate the presence and concentration of intra-synovial inflammatory cytokines in CAPJs with and without standing cone beam CT evidence of OA using an equine specific multiplex assay.
Prospective clinical study.
Standing cone beam CT of C5-6 and C6-7 was performed on horses with CAPJ OA and control horses. Synovial fluid samples of the CAPJs of C5-6 and C6-7 were obtained bilaterally using ultrasound guidance and analysed for concentrations of IFN-γ, IL-1β, IL-6, IL-10, IL-17 and TNFα with the Milliplex® multi-analyte profiling kit. CT Images were retrospectively graded using a novel grading scheme. Significant differences between concentrations of inflammatory cytokines between joints with different categories of osteoarthritis severity were explored using a Wilcoxon rank-sum test or Kruskal-Wallis test.
Concentrations of intra-synovial cytokines were higher in joints with moderate to severe OA when compared to joints with no or mild OA, with differences in concentrations of IL-17 reaching statistical significance (P = .007).
Limitations include discrepancy in number, age, and breed between control and OA populations, use of a novel grading scheme, and lack of a histologic gold-standard to confirm the presence and severity of CAPJ OA.
Differences in inflammatory cytokines between caudal CAPJs with and without evidence of moderate to severe osteoarthritis on standing cone beam CT exist. This finding lends support to the clinical relevance of a diagnosis of moderate to severe CAPJ OA in the caudal cervical vertebral column as identified with this imaging modality.
站立锥形束 CT 可对镇静马的尾颈椎关节突关节 (CAPJ) 进行横断面成像，尽管在该部位 CT 诊断的骨关节炎 (OA) 的临床意义尚不清楚。滑液中细胞因子浓度的增加可能支持在该影像学方式上诊断的 CAPJ OA 的临床意义。
使用马专用的多重分析试剂盒，研究 CAPJ 有无站立锥形束 CT 所示 OA 时关节内炎症细胞因子的存在和浓度。
前瞻性临床研究。
对 CAPJ OA 马和对照马进行 C5-6 和 C6-7 的站立锥形束 CT。使用超声引导从双侧 C5-6 和 C6-7 的 CAPJ 获得滑液样本，并使用 Milliplex®多分析物分析试剂盒分析 IFN-γ、IL-1β、IL-6、IL-10、IL-17 和 TNFα 的浓度。使用新的分级方案对 CT 图像进行回顾性分级。使用 Wilcoxon 秩和检验或 Kruskal-Wallis 检验探索不同 OA 严重程度关节之间炎症细胞因子浓度的差异。
与无或轻度 OA 相比，中重度 OA 关节的关节内细胞因子浓度更高，IL-17 浓度的差异具有统计学意义 (P=.007)。
局限性包括对照组和 OA 组在数量、年龄和品种上的差异，使用新的分级方案，以及缺乏组织学金标准来确认 CAPJ OA 的存在和严重程度。
站立锥形束 CT 显示有或无中重度 CAPJ OA 的尾 CAPJ 之间的炎症细胞因子存在差异。这一发现支持在该影像学方式上诊断的中重度尾颈椎 CAPJ OA 的临床相关性。
