Sánchez-Fauquier A, Villanueva N, Melero J A
Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
Arch Virol. 1987;97(3-4):251-65. doi: 10.1007/BF01314425.
The large (HA1) and small (HA2) subunits of influenza virus A/Vict/3/75 hemagglutinin were purified in denatured form by preparative electrophoresis. Both polypeptides were used to immunize mice from which monoclonal antibodies were obtained. These antibodies reacted not only with the corresponding hemagglutinin subunit but also with purified virions. When tested by radioimmunoassay against a panel of human viruses, most anti-HA1 and -HA2 antibodies behaved as subtype-specific, whereas anti-HA antibodies, raised against purified virus, were more restricted. The anti-subunit antibodies were negative in hemagglutination-inhibition and neutralization tests. The interest of these antibodies as reagents for research and diagnosis is discussed.
通过制备电泳以变性形式纯化了甲型流感病毒A/Vict/3/75血凝素的大亚基（HA1）和小亚基（HA2）。使用这两种多肽免疫小鼠，从而获得单克隆抗体。这些抗体不仅与相应的血凝素亚基发生反应，还与纯化的病毒粒子发生反应。当通过放射免疫测定法针对一组人类病毒进行测试时，大多数抗HA1和抗HA2抗体表现为亚型特异性，而针对纯化病毒产生的抗HA抗体的特异性更强。抗亚基抗体在血凝抑制试验和中和试验中呈阴性。讨论了这些抗体作为研究和诊断试剂的意义。
