Green N, Alexander H, Olson A, Alexander S, Shinnick T M, Sutcliffe J G, Lerner R A
Cell. 1982 Mar;28(3):477-87. doi: 10.1016/0092-8674(82)90202-1.
We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but rather are scattered throughout its three-dimensional structure. In contrast, antibody raised to intact hemagglutinin did not react with any of the 20 peptides. Taken together these results suggest that the immunogenicity of an intact protein molecule is not the sum of the immunogenicity of its pieces.
我们化学合成了20种与流感病毒HA1分子75%相对应的肽段。针对其中大多数（18种）肽段产生的抗体能够与血凝素分子发生反应。这18种肽段并不局限于血凝素分子已知的抗原决定簇，而是散布在其三维结构中。相比之下，针对完整血凝素产生的抗体与这20种肽段中的任何一种都不发生反应。综合这些结果表明，完整蛋白质分子的免疫原性并非其各个片段免疫原性的总和。
