肺炎克雷伯菌固氮酶D基因的核苷酸序列及固氮酶钼铁蛋白α亚基的预测氨基酸序列。
Nucleotide sequence of the Klebsiella pneumoniae nifD gene and predicted amino acid sequence of the alpha-subunit of nitrogenase MoFe protein.
作者信息
Ioannidis I, Buck M
机构信息
A.F.R.C. Unit of Nitrogen Fixation, University of Sussex, Brighton, U.K.
出版信息
Biochem J. 1987 Oct 15;247(2):287-91. doi: 10.1042/bj2470287.
Abstract
The nucleotide sequence of the Klebsiella pneumoniae nifD gene is presented and together with the accompanying paper [Holland, Zilberstein, Zamir & Sussman (1987) Biochem. J. 247, 277-285] completes the sequence of the nifHDK genes encoding the nitrogenase polypeptides. The K. pneumoniae nifD gene encodes the 483-amino acid-residue nitrogenase alpha-subunit polypeptide of Mr 54156. The alpha-subunit has five strongly conserved cysteine residues at positions 63, 89, 155, 184 and 275, some occurring in a region showing both primary sequence and potential structural homology to the K. pneumoniae nitrogenase beta-subunit. A comparison with six other alpha-subunit amino acid sequences has been made, which indicates a number of potentially important domains within alpha-subunits.
摘要
本文给出了肺炎克雷伯菌nifD基因的核苷酸序列，与随附论文[霍兰德、齐尔伯斯坦、扎米尔和苏斯曼（1987年）《生物化学杂志》247卷，277 - 285页]共同完成了编码固氮酶多肽的nifHDK基因序列。肺炎克雷伯菌nifD基因编码Mr 54156的483个氨基酸残基的固氮酶α亚基多肽。α亚基在第63、89、155、184和275位有五个高度保守的半胱氨酸残基，其中一些位于与肺炎克雷伯菌固氮酶β亚基显示出一级序列和潜在结构同源性的区域。已与其他六个α亚基氨基酸序列进行了比较，这表明α亚基内有一些潜在的重要结构域。
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本文引用的文献
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