肺炎克雷伯氏菌nifV突变体的固氮酶含有一种铁钼辅因子的变体形式。
Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor.
作者信息
Hawkes T R, McLean P A, Smith B E
出版信息
Biochem J. 1984 Jan 1;217(1):317-21. doi: 10.1042/bj2170317.
Abstract
When the iron-molybdenum cofactor (FeMoco) was extracted from the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae and combined with the FeMoco-deficient MoFe protein from a nifB mutant, the resultant MoFe protein exhibited the NifV phenotype, i.e. in combination with wild-type Fe protein it exhibited poor N2-fixation activity and its H2-evolution activity was inhibited by CO. These data provide strong evidence that FeMoco contains the active site of nitrogenase. The metal contents and e.p.r. properties of FeMoco from wild-type and nifV mutants of K. pneumoniae are very similar.
摘要
当从肺炎克雷伯氏菌nifV突变体的固氮酶钼铁蛋白中提取铁钼辅因子（FeMoco），并与来自nifB突变体的缺乏FeMoco的钼铁蛋白结合时，所得的钼铁蛋白表现出NifV表型，即与野生型铁蛋白结合时，其固氮活性较差，并且其放氢活性受到CO的抑制。这些数据提供了强有力的证据，证明FeMoco含有固氮酶的活性位点。肺炎克雷伯氏菌野生型和nifV突变体的FeMoco的金属含量和电子顺磁共振特性非常相似。
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1
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Nature. 1981 Aug 13;292(5824):655-6. doi: 10.1038/292655a0.
2
Large-scale purification of high activity Azotobacter vinelandII nitrogenase.维涅兰德固氮菌高活性固氮酶的大规模纯化
Biochim Biophys Acta. 1980 Jul 10;614(1):196-209. doi: 10.1016/0005-2744(80)90180-1.
3
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Proc Natl Acad Sci U S A. 1983 Jan;80(1):147-50. doi: 10.1073/pnas.80.1.147.
4
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Biochem J. 1983 Jun 1;211(3):589-97. doi: 10.1042/bj2110589.
5
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Biochem J. 1983 Jan 1;209(1):43-50. doi: 10.1042/bj2090043.
6
Iron-molybdenum cofactor from nitrogenase. Modified extraction methods as probes for composition.来自固氮酶的铁钼辅因子。改良提取方法作为成分探测手段。
J Biol Chem. 1982 Jul 25;257(14):8042-8.
7
Isolation of a molybdenum--iron cluster from nitrogenase.从固氮酶中分离出钼铁簇。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3438-40. doi: 10.1073/pnas.78.6.3438.
8
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Biochim Biophys Acta. 1980 May 29;623(1):124-38. doi: 10.1016/0005-2795(80)90015-x.
9
Studies by electron paramagnetic resonance on the catalytic mechanism of nitrogenase of Klebsiella pneumoniae.肺炎克雷伯菌固氮酶催化机制的电子顺磁共振研究。
Biochem J. 1973 Oct;135(2):331-41. doi: 10.1042/bj1350331.
10
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Biochem J. 1972 Nov;130(1):239-49. doi: 10.1042/bj1300239.
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