Sabel'nikov A G, Moiseeva T F, Avdeeva A V, Il'iashenko B N
Biofizika. 1977 Jul-Aug;22(4):640-5.
The interaction of ANS with Escherichia coli cells, isolated cell walls, total cell envelopes and inner membranes was investigated in the presence and absence of Ca2+ ions. The addition of Ca2+ to intact cells at room temperature did not result in enhancement of fluorescence intensity. On the contrary the addition of Ca2+ to intact cells at 2 degrees C resulted in a progressive increase of fluorescence intensity with the maximum reached approximately by the 20th minute. The addition of Ca2+ to different membrane preparations showed a two-fold increase. Addition of Ca2+ to spheroplast membrane preparations did not result in any increase of the number of binding sites for ANS. There was a four-fold increase in quantum yields. All membrane preparations in titrations with Ca2+ showed saturation kinetics. The obtained results are discussed in terms of structural alterations in E. coli membranes and in relation with biological effects.
在存在和不存在钙离子的情况下，研究了ANS与大肠杆菌细胞、分离的细胞壁、完整细胞包膜和内膜的相互作用。在室温下向完整细胞中添加钙离子不会导致荧光强度增强。相反，在2℃下向完整细胞中添加钙离子会导致荧光强度逐渐增加，大约在第20分钟达到最大值。向不同的膜制剂中添加钙离子显示增加了两倍。向原生质球膜制剂中添加钙离子不会导致ANS结合位点数量的任何增加。量子产率增加了四倍。在用钙离子进行滴定的所有膜制剂中均显示出饱和动力学。根据大肠杆菌膜的结构变化以及与生物学效应的关系对所得结果进行了讨论。
