Sabel'nikov A G, Il'iashenko B N
Biofizika. 1978 Jul-Aug;23(4):730-2.
Using an uncharged hydrophobic fluorescent probe 4-dimethylaminochalkon (DMC) the thermotropic behaviour of cytoplasmic E. coli membranes in the presence of Ca2+ cations was investigated. DMC appeared to be very sensitive in detection of temperature induced phase transitions in E. coli membrane preparations. It was found unexpectedly that Ca2+ does not increased the phase transition temperature of membrane preparations, compared to the values calculated for the membranes in tris-HC1-buffer. The obtained results are briefly discussed.
使用不带电荷的疏水性荧光探针4-二甲基氨基查耳酮（DMC），研究了在Ca2+阳离子存在下大肠杆菌细胞质膜的热致行为。DMC在检测大肠杆菌膜制剂中温度诱导的相变方面似乎非常敏感。意外地发现，与在tris-HCl缓冲液中计算的膜的值相比，Ca2+并没有提高膜制剂的相变温度。对所得结果进行了简要讨论。
