Department of Microbiology, Kamaraj College, Thoothukudi, India.
Department of Zoology, Kamaraj College, Thoothukudi, India.
Archaea. 2020 Nov 9;2020:8844811. doi: 10.1155/2020/8844811. eCollection 2020.
Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel . A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV. There were 16 mutant strains exhibiting chitinase activity. Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as (SSCL-10) with the highest specific activity of 3.4 U/mL. The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain. The partially purified chitinase has a molecular weight of 66 kDa. The wild strain (SSCL-10) was identified as using 16S rRNA sequence analysis. This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.
几丁质酶或几丁质裂解酶在医学、农业和工业领域有不同的应用。本研究旨在开发一种新型的高效产超几丁质酶的突变菌株。本研究采用乙磺酸乙酯化学诱变和紫外线照射两种简单快速的方法，筛选潜在的几丁质裂解微生物群。共筛选到 16 株具有几丁质酶活性的突变株。在产几丁质酶的菌株中，选择了几丁质酶活性最高的菌株，用 SDS-PAGE 对该酶进行了部分纯化，并鉴定为 （SSCL-10），其比活力最高，为 3.4 U/mL。成功地在突变株 EMS-13（20.2 U/mL）中实现了诱导突变模型，该突变株产生的几丁质酶产量提高了 5-6 倍，而突变株 UV-11（13.3 U/mL）的几丁质酶活性比野生型提高了 3-4 倍。部分纯化的几丁质酶的分子量为 66 kDa。通过 16S rRNA 序列分析，将野生株（SSCL-10）鉴定为 。本研究探讨了高产几丁质酶细菌在甲壳类和虾类甲壳素废物回收和处理方面的潜在应用，从而为甲壳类产业增加了价值。
