Snoeck Paola Pereira das Neves, Pessoa Tainá Hortência Oliveira, Pereira Marcelo Gonçalves Sousa, Bastos Inara Cristina Leal, de Melo Maria Isabel Vaz
Universidade Estadual de Santa Cruz, Ciências Agrárias e Ambientais, Ilheus, Bahia, Brazil.
Pontífica Universidade Católica de Minas Gerais, Medicina Veterinária, Belo Horizonte, Brazil.
Anim Reprod. 2019 Oct 23;16(2):340-347. doi: 10.21451/1984-3143-AR2019-0039.
The objective of this study was to compare the BotuCrio extender with the Merk - egg yolk and the INRA 82 modified by the inclusion of acetamide, methyl cellulose and trehalose in substitution of glycerol for freezing equine semen. The semen was diluted after centrifugation to obtain 100 x 10 of sperm/ml in: BotuCrio (control); Merk - egg yolk or INRA 82 modified (Experiment 1). The extended semen was packaged in 0.5 ml straws, cooled and frozen in a freezing machine. The control extender was superior in preserving the motility, VCL, VSL, VAP, LIN, STR and the BCF when compared to the Merk - egg yolk and INRA 82 modified (P < 0.05). The BotuCrio® preserved more effectively the equine sperm viability characteristics evaluated in Experiment 1 and was used as a control extender in Experiment 2 to test the effectiveness of using LDL in replacement of egg yolk. BotuCrio was superior to preserve progressive motility, VCL, VSL, VAP, LIN, STR and the percentage of functional integrity of sperm membranes compared to BotuCrio LDL (P < 0.05). However, both extenders preserved similarly the total motility, ALH, BCF and the structural integrity of the membranes (P > 0.05). The fertility rate after AI with frozen semen in BotuCrio LDL was 37.5%.
本研究的目的是比较BotuCrio稀释液与Merk - 蛋黄稀释液以及通过添加乙酰胺、甲基纤维素和海藻糖替代甘油对INRA 82进行改良后的稀释液，用于冷冻马精液。精液离心后进行稀释，使其在BotuCrio（对照）、Merk - 蛋黄或改良后的INRA 82（实验1）中达到100×10⁶精子/ml。将稀释后的精液包装在0.5 ml细管中，冷却并在冷冻机中冷冻。与Merk - 蛋黄和改良后的INRA 82相比，对照稀释液在保存精子活力、曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）、线性度（LIN）、摆动性（STR）和鞭打频率（BCF）方面更具优势（P < 0.05）。BotuCrio®在实验1中更有效地保存了所评估的马精子活力特征，并在实验2中用作对照稀释液，以测试用低密度脂蛋白（LDL）替代蛋黄的有效性。与BotuCrio LDL相比，BotuCrio在保存精子的前向运动能力、VCL、VSL、VAP、LIN、STR和精子膜功能完整性百分比方面更具优势（P < 0.05）。然而，两种稀释液在保存总活力、平均振幅侧摆（ALH）、BCF和膜的结构完整性方面相似（P > 0.05）。使用BotuCrio LDL冷冻精液进行人工授精后的受胎率为37.5%。
