Petersen Jeffrey, Dalal Sharvari, Jhala Darshana
Corporal Michael J. Crescenz Veteran Affairs Medical Center, Philadelphia, Pennsylvania.
University of Pennsylvania, Philadelphia, Pennsylvania.
Lab Med. 2021 Mar 15;52(2):e39-e45. doi: 10.1093/labmed/lmaa099.
With the COVID-19 pandemic, there have been supply challenges necessitating that laboratories must prepare their own viral transport medium (VTM), which provides stability for clinical specimens for diagnostic viral testing.
Within a veteran affairs medical center clinical laboratory, VTM was prepared with a Hanks Balanced Salt Solution (HBSS) 500 mL bottle with phenol red, sterile heat-inactivated fetal bovine serum (FBS), gentamicin sulfate (50 mg/mL), and amphotericin B (250 μg/mL). An antimicrobial mixture was made of 50 mL each of amphotericin B and gentamicin sulfate. Ten mL of FBS and 2 mL of the antimicrobial mixture were mixed into the HBSS bottle, from which 3 mL aliquots were made. Sterility and efficacy check were assessed. These preparations were conducted at our VAMC's clinical laboratory to assure adequate VTM supply during the COVID-19 shortage.
The VTM was successfully prepared in-house, supporting uninterrupted testing for the facility and other affiliated medical facilities/centers and community living centers.
This quality assurance/improvement report represents the first published manuscript on feasible VTM preparation exclusively within a clinical microbiology laboratory during the COVID-19 pandemic.
在新冠疫情期间，出现了供应方面的挑战，这使得实验室必须自行制备病毒运输培养基（VTM），该培养基可为用于诊断病毒检测的临床标本提供稳定性。
在一家退伍军人事务医疗中心的临床实验室中，用含有酚红的500毫升汉克斯平衡盐溶液（HBSS）瓶、无菌热灭活胎牛血清（FBS）、硫酸庆大霉素（50毫克/毫升）和两性霉素B（250微克/毫升）制备VTM。将两性霉素B和硫酸庆大霉素各50毫升制成抗菌混合物。将10毫升FBS和2毫升抗菌混合物加入到HBSS瓶中，从中取出3毫升分装。评估无菌性和有效性。这些制备工作在我们退伍军人事务医疗中心的临床实验室进行，以确保在新冠疫情期间VTM的充足供应。
VTM成功在内部制备完成，为该机构以及其他附属医疗设施/中心和社区生活中心的检测工作提供了不间断支持。
这份质量保证/改进报告是首篇发表的关于在新冠疫情期间仅在临床微生物实验室中成功制备可行的VTM的手稿。
