Dutton G G, Kuma-Mintah A
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Carbohydr Res. 1987 Nov 15;169:213-20. doi: 10.1016/0008-6215(87)80252-5.
The structure of the heat-stable K antigen of Escherichia coli serotype K34 has been determined by n.m.r. spectroscopy, methylation analysis, and Smith degradation to be (formula; see text) The assignment of the single alpha-linkage to the D-glucosyl residue was confirmed by the positive reaction of an alpha-D-glucosidase on the oligosaccharide obtained by bacteriophage depolymerization. It is noteworthy that the same enzyme was without action on the native polysaccharide.
通过核磁共振光谱、甲基化分析和史密斯降解法确定了大肠杆菌血清型K34的热稳定K抗原结构为（分子式；见正文）。通过α-D-葡萄糖苷酶对噬菌体解聚得到的寡糖的阳性反应，证实了单个α-连接与D-葡萄糖基残基的归属。值得注意的是，同一种酶对天然多糖没有作用。
