Sturgess M L, Weeks I, Evans P J, Mpoko C N, Laing I, Woodhead J S
Department of Medical Biochemistry and Medicine, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Clin Endocrinol (Oxf). 1987 Sep;27(3):383-93. doi: 10.1111/j.1365-2265.1987.tb01165.x.
An immunochemiluminometric assay has been developed for the measurement of free T4 concentrations in serum. The assay uses chemiluminescent acridinium ester labelled monoclonal antibodies which react with free T4 in the sample. A T4-rabbit immunoglobulin G conjugate competes for antibody binding sites, immune-complexes containing this being isolated using an anti-immunoglobulin G antibody coupled to paramagnetic particles. Associated chemiluminescence intensity is thus dependent on the free T4 concentration. The assay distinguishes patients with primary thyroid disease from euthyroid subjects and is unaffected by abnormal binding proteins which compromise the diagnostic accuracy of radiolabelled analogue immunoassays. the test yields results which accurately reflect the clinical thyroid status of euthyroid patients with a variety of acute and chronic non-thyroid illnesses. This is again in marked contrast to the aberrant results seen using certain radiolabelled analogue procedures.
已开发出一种免疫化学发光分析法，用于测定血清中游离T4的浓度。该分析方法使用化学发光吖啶酯标记的单克隆抗体，其与样品中的游离T4发生反应。一种T4 - 兔免疫球蛋白G结合物竞争抗体结合位点，含有该结合物的免疫复合物使用与顺磁性颗粒偶联的抗免疫球蛋白G抗体进行分离。因此，相关的化学发光强度取决于游离T4的浓度。该分析方法可区分原发性甲状腺疾病患者和甲状腺功能正常的受试者，并且不受异常结合蛋白的影响，而这些异常结合蛋白会影响放射性标记类似物免疫分析的诊断准确性。该检测产生的结果能够准确反映患有各种急性和慢性非甲状腺疾病的甲状腺功能正常患者的临床甲状腺状态。这再次与使用某些放射性标记类似物方法所得到的异常结果形成鲜明对比。
