Sturgess M L, Weeks I, Mpoko C N, Laing I, Woodhead J S
Clin Chem. 1986 Mar;32(3):532-5.
A chemiluminescent labeled-antibody immunoassay has been developed for measurement of total thyroxin (T4) in serum. Monoclonal antibodies to T4 labeled with a chemiluminescent acridinium ester are used. Serum samples are incubated with the labeled antibodies and a thyroxin-rabbit immunoglobulin conjugate, then reacted with magnetizable particles containing sheep anti-rabbit immunoglobulin. The total reaction time is 40 min. The chemiluminescence intensity of the solid-phase immune complexes is inversely proportional to the concentration of T4 in the sample. The sensitivity of the assay is 1 nmol/L, and the working range of 20-190 nmol/L is characterized by CVs less than or equal to 10%.
已开发出一种化学发光标记抗体免疫测定法，用于测定血清中的总甲状腺素（T4）。使用用化学发光吖啶酯标记的T4单克隆抗体。血清样本与标记抗体和甲状腺素-兔免疫球蛋白缀合物一起孵育，然后与含有羊抗兔免疫球蛋白的可磁化颗粒反应。总反应时间为40分钟。固相免疫复合物的化学发光强度与样本中T4的浓度成反比。该测定法的灵敏度为1 nmol/L，在20 - 190 nmol/L的工作范围内，变异系数（CV）小于或等于10%。
