Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey.
Trop Anim Health Prod. 2020 Nov 23;53(1):28. doi: 10.1007/s11250-020-02428-x.
The objective of this study was to determine the efficacy of the Fassisi®BoviPreg visual test kit (Fassisi®BoviPreg) in determining early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in whole blood and blood serum. The study was conducted on 50 cows, the artificial insemination (AI) dates of which were designated as day 0. Pregnancy diagnosis was performed with transrectal ultrasonography (USG), and serum samples were simultaneously collected and used with Fassisi®BoviPreg to determine pregnancies on 30 days after AI. The results of the Fassisi®BoviPreg test on serum and whole blood samples, respectively, on 30 days after AI were as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; positive predictive values, 76.2% and 68.4%; negative predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%. On day 50 after AI, sensitivities were 63.64% and 50.0%, specificities were 100.0% and 100.0%, and accuracies were 75.0% and 65.62% in serum and whole blood, respectively. Higher pregnancy rates were obtained using Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the results from the Fassisi®BoviPreg tests on cows on 30 and 50 days after AI showed that the use of blood serum is more accurate and suitable than that of whole blood. The results also showed a higher confidence level in specificity 50 days after AI.
本研究旨在通过测量全血和血清中的妊娠相关糖蛋白（PAG）来确定 Fassisi®BoviPreg 视觉检测试剂盒（Fassisi®BoviPreg）在牛早期妊娠中的功效。该研究共涉及 50 头奶牛，人工授精（AI）日期被指定为第 0 天。通过直肠超声（USG）进行妊娠诊断，并同时采集血清样本，使用 Fassisi®BoviPreg 在 AI 后 30 天进行妊娠诊断。AI 后 30 天，血清和全血 Fassisi®BoviPreg 检测结果如下：血清样本灵敏度分别为 61.54%和 50.0%，特异性分别为 79.17%和 75.0%，准确率分别为 70.0%和 62.0%，阳性预测值分别为 76.2%和 68.4%，阴性预测值分别为 65.5%和 58.1%，假阳性诊断率分别为 23.8%和 31.6%，假阴性诊断率分别为 34.5%和 41.9%。AI 后第 50 天，血清和全血的灵敏度分别为 63.64%和 50.0%，特异性分别为 100.0%和 100.0%，准确率分别为 75.0%和 65.62%。在 P4≥5ng/mL 的牛中使用 Fassisi®BoviPreg 时，妊娠率更高（P<0.001）。总之，AI 后 30 天和 50 天 Fassisi®BoviPreg 检测牛的结果表明，血清的使用比全血更准确、更合适。结果还表明，AI 后 50 天特异性的置信度更高。
