Safak Tarik, Tümer Kenan Çağrı, Alp Yağmur İpek, Özen Taha Yasin, Yilmaz-Koc Oznur
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey.
Department of Internal Medicine, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey.
Vet Med Sci. 2025 Mar;11(2):e70226. doi: 10.1002/vms3.70226.
The aim of this study was to determine and compare the diagnostic accuracies of two commercial pregnancy-associated glycoprotein tests, Alertys OnFarm Pregnancy Test (AOPT) and Alertys Milk Pregnancy Test (AMPT), for early pregnancy diagnosis in dairy cattle. Holstein cows (n = 124) were used in the study. Whole blood samples were collected from the jugular vein 28 days after fixed-time artificial insemination (FTAI). In addition, teats of these cows were swabbed and milk samples were collected into sterile Falcon tubes. AOPT was performed on the farm within 2 h after whole blood collection. Milk samples for AMPT analysis were taken to the laboratory and analysed within 2 h. Transrectal ultrasonography was performed on the 32nd day after FTAI as a reference test. Comparative evaluation was made according to the AOPT and AMPT results, 28 days after FTAI. The sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) for the AOPT were 92.4%, 80.0%, 87.9%, 89.0% and 85.7% and for AMPT were 97.5%, 82.2%, 91.9%, 90.6% and 94.9% respectively. Cohen's kappa statistic showed a 91.9% agreement (kappa = 0.820, p < 0.001) between the reference test and AMPT, and an 87.9% agreement (kappa = 0.735, p < 0.001) between the reference test and AOPT. AOPT and AMPT offers a reliable, non-invasive (in milk) and practical approach to pregnancy diagnosis in cows. These methods enable early pregnancy detection and can be easily integrated into farm routines, enhancing reproductive management and overall herd productivity.
本研究的目的是确定并比较两种商业化的妊娠相关糖蛋白检测方法，即Alertys OnFarm妊娠检测（AOPT）和Alertys牛奶妊娠检测（AMPT），用于奶牛早期妊娠诊断的诊断准确性。本研究使用了荷斯坦奶牛（n = 124）。在定时人工授精（FTAI）后28天从颈静脉采集全血样本。此外，擦拭这些奶牛的乳头，并将牛奶样本收集到无菌的Falcon管中。全血采集后2小时内在农场进行AOPT检测。用于AMPT分析的牛奶样本被送到实验室并在2小时内进行分析。在FTAI后第32天进行经直肠超声检查作为参考检测。根据FTAI后28天的AOPT和AMPT结果进行比较评估。AOPT的敏感性、特异性、准确性、阳性预测值（PPV）和阴性预测值（NPV）分别为92.4%、80.0%、87.9%、89.0%和85.7%，AMPT的分别为97.5%、82.2%、91.9%、90.6%和94.9%。Cohen's kappa统计量显示参考检测与AMPT之间的一致性为91.9%（kappa = 0.820，p < 0.001），参考检测与AOPT之间的一致性为87.9%（kappa = 0.735，p < 0.001）。AOPT和AMPT为奶牛妊娠诊断提供了一种可靠、非侵入性（在牛奶中）且实用的方法。这些方法能够早期检测妊娠，并且可以轻松融入农场日常工作中，加强繁殖管理并提高整个牛群的生产力。
