诱导气道对卵清蛋白和尘螨变应原的过敏反应作为变应性哮喘的小鼠模型。

Induction of Airway Hypersensitivity to Ovalbumin and Dust Mite Allergens as Mouse Models of Allergic Asthma.

机构信息

Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA.

出版信息

Methods Mol Biol. 2021;2223:101-114. doi: 10.1007/978-1-0716-1001-5_8.

Abstract

Mouse models of allergic asthma have been utilized to establish the role of T helper type 2 (Th2) cells in driving lung inflammation, airway hyperresponsiveness, and obstruction. Here, we present the allergic asthma models, in which mice are hypersensitized to ovalbumin (OVA) and house dust mite (HDM). These models mimic the major characteristics of human asthma including the eosinophilic inflammation and hyperactivity of the airway, overproduction of Th2 cytokines in the lung, and elevated total and allergen-specific immunoglobulin E (IgE) in serum.

摘要

已利用过敏性哮喘的小鼠模型来确定辅助性 T 细胞 2 型（Th2）在驱动肺部炎症、气道高反应性和阻塞中的作用。在这里，我们介绍了过敏性哮喘模型，其中小鼠对卵清蛋白（OVA）和屋尘螨（HDM）过敏。这些模型模拟了人类哮喘的主要特征，包括嗜酸性粒细胞炎症和气道高反应性、肺部 Th2 细胞因子过度产生以及血清总免疫球蛋白 E（IgE）和过敏原特异性 IgE 升高。