Therapeutic Innovation Lab, and Laboratory of Excellence Medalis, UMR7200 CNRS-Strasbourg University, Illkirch, France.
UMS3286 CNRS-Strasbourg University, PCBIS Platform, Illkirch, France.
Methods Mol Biol. 2021;2241:75-87. doi: 10.1007/978-1-0716-1095-4_7.
Murine models of asthma are developed to better understand the mechanisms of asthma including eosinophil recruitment in the airways with the aim of evaluating new therapeutic strategies. They are intended to model the typical features of human disease, in particular airway inflammation, hyperresponsiveness (AHR), and remodeling. The phenotype of inflammatory cells recovered from the bronchoalveolar lavage fluid (BAL) is studied with innovative flow cytometry techniques while airway obstruction is measured using the forced oscillation technique, and airway responsiveness approached by barometric plethysmography in awake and unconstrained animals. We here describe models of asthma of house dust mite (HDM) as a clinically relevant allergen: a short study design (8 days) model of hypereosinophilic asthma and a chronic (31 days) asthma model, both suitable to evaluate the potential of new drug candidates to prevent allergic asthma.
哮喘的小鼠模型被开发出来，以更好地了解哮喘的机制，包括气道中嗜酸性粒细胞的募集，目的是评估新的治疗策略。它们旨在模拟人类疾病的典型特征，特别是气道炎症、高反应性（AHR）和重塑。通过创新的流式细胞术技术研究从支气管肺泡灌洗液（BAL）中回收的炎症细胞的表型，而使用强迫振荡技术测量气道阻塞，并在清醒和不受约束的动物中通过体描仪测量气道反应性。在这里，我们描述了屋尘螨（HDM）作为一种临床相关过敏原的哮喘模型：一种短期（8 天）高嗜酸性粒细胞性哮喘模型和一种慢性（31 天）哮喘模型，都适合评估新候选药物预防过敏性哮喘的潜力。
