豚草和木瓜蛋白酶诱导的过敏性结膜炎的实验小鼠模型。

Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis.

机构信息

Laboratory of Ocular Atopic Diseases, Department of Ophthalmology, Juntendo University Graduate School of Medicine, Tokyo, Japan.

Laboratory of System Biology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

出版信息

Methods Mol Biol. 2021;2223:133-149. doi: 10.1007/978-1-0716-1001-5_10.

DOI:10.1007/978-1-0716-1001-5_10

PMID:33226592

Abstract

Mouse models of allergic conjunctivitis mimic various aspects of human allergic conjunctivitis. They are useful as acute models of allergic conjunctivitis to study immunological aspects of this condition. In this chapter, we will describe ragweed-pollen-induced experimental allergic conjunctivitis (mostly driven by adaptive immunity), and papain-soaked contact lens-induced experimental allergic conjunctivitis (mostly driven by innate immunity). Giemsa staining of histological sections is used for quantification of the number of infiltrating eosinophils, which is useful to evaluate the severity of the allergic inflammation. Immunohistochemical staining and quantitative PCR are used to clarify spatiotemporal expression of proinflammatory molecules in the conjunctival tissue. Flow cytometric analysis of conjunctival tissue is used for the detection of innate lymphoid cell type 2 (ILC2) in the ocular surface tissues.

摘要

过敏性结膜炎的小鼠模型模拟了人类过敏性结膜炎的各个方面。它们可用作过敏性结膜炎的急性模型，用于研究这种疾病的免疫学方面。在本章中，我们将描述豚草花粉诱导的实验性过敏性结膜炎（主要由适应性免疫驱动）和木瓜蛋白酶浸泡隐形眼镜诱导的实验性过敏性结膜炎（主要由固有免疫驱动）。对组织学切片进行吉姆萨染色用于定量浸润嗜酸性粒细胞的数量，这有助于评估过敏炎症的严重程度。免疫组织化学染色和定量 PCR 用于阐明结膜组织中促炎分子的时空表达。对结膜组织进行流式细胞术分析用于检测眼表面组织中的 2 型固有淋巴细胞（ILC2）。