Department of Surgery, Davis Heart and Lung Research Institute, Center for Regenerative Medicine & Cell-Based Therapies, The Ohio State University Wexner Medical Center, Columbus, OH, United States of America.
Department of Surgery, IUH Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, IN, United States of America.
PLoS One. 2020 Nov 23;15(11):e0241831. doi: 10.1371/journal.pone.0241831. eCollection 2020.
Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.
为了了解组织修复的连续性，需要对同一伤口进行非侵入性、重复的检测。在这项工作中，我们试图测试非侵入性高频高分辨率超声技术对此类检测的意义。采用高频高分辨率超声成像技术研究胎儿和成人条件下的伤口愈合。使用 Vevo 应变软件获得定量组织细胞密度和弹性应变，以可视化未解决的炎症。使用彩色多普勒血流成像研究供应伤口部位的动脉血流的血液动力学特性。非侵入性监测胎儿和成人伤口愈合提供了前所未有的生物力学和功能见解。胎儿伤口表现出高度加速的闭合，伴有伤口组织细胞密度的短暂波动。胎儿血流动力学是独特的，动脉脉搏压 (APP) 急剧下降，在受伤后 48 小时内迅速恢复。在成年人中，APP 在受伤后短暂增加，然后在受伤后第 10 天恢复到受伤前的水平。糖尿病患者伤口边缘组织弹性变化的模式明显不同。在炎症早期获得的严重应变持续存在，与非糖尿病组相比，弹性恢复较慢。与同窝对照（db/+）相比，成年糖尿病小鼠（db/db）的伤口床表现出持续的高细胞密度，表明炎症持续时间较长。db/+ 和 db/db 的正常皮肤应变不同步。在 db/db 中，在炎症早期获得的严重应变持续存在，与非糖尿病患者相比，弹性恢复较慢。本研究展示了一种适用于实时分析功能性伤口愈合的多功能临床相关成像平台。
