Material Health Science Laboratory, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei-shi, Tokyo, 184-8588, Japan.
Faculty of Health Sciences, Department of Physical Therapy, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo, 181-8612, Japan.
Cell Tissue Res. 2019 Apr;376(1):123-135. doi: 10.1007/s00441-018-2954-3. Epub 2018 Nov 17.
Signs of aging in facial skin correlate with lifespan and chronic disease; however, the health of aging skin has not been extensively studied. In healthy young skin, the dermis forms a type III collagen-rich dermal papilla, where capillary vessels supply oxygen and nutrients to basal epidermal cells. Chicken eggshell membranes (ESMs) have been used as traditional medicines to promote skin wound healing in Asian countries for many years. Previously, we designed an experimental system in which human dermal fibroblasts (HDFs) were cultured on a dish with a solubilized ESM (S-ESM) bound to an artificial phosphorylcholine polymer; we found that genes that promoted the health of the papillary dermis, such as those encoding type III collagen, were induced in the S-ESM environment. The present study found that a gel with a ratio of 20% type III/80% type I collagen, similar to that of the baby skin, resulted in a higher elasticity than 100% type I collagen (p < 0.05) and that HDFs in the gel showed high mitochondrial activity. Thus, we decided to perform further evaluations to identify the effects of S-ESM on gene expression in the skin of hairless mice and found a significant increase of type III collagen in S-ESM. Picrosirius Red staining showed that type III collagen significantly increased in the papillary dermis after S-ESM treatment. Moreover, S-ESM application significantly improved human arm elasticity and reduced facial wrinkles. ESMs may have applications in extending lifespan by reducing the loss of tissue elasticity through the increase of type III collagen.
面部皮肤的衰老迹象与寿命和慢性疾病有关；然而，衰老皮肤的健康状况尚未得到广泛研究。在健康的年轻皮肤中，真皮形成富含 III 型胶原蛋白的真皮乳头，毛细血管为基底层表皮细胞提供氧气和营养。鸡卵壳膜 (ESM) 多年来一直被亚洲国家用作传统药物来促进皮肤伤口愈合。先前，我们设计了一种实验系统，其中人类真皮成纤维细胞 (HDF) 在与人工磷酸胆碱聚合物结合的可溶 ESM (S-ESM) 覆盖的培养皿上培养；我们发现，在 S-ESM 环境中诱导了促进乳头真皮健康的基因，例如编码 III 型胶原蛋白的基因。本研究发现，类似于婴儿皮肤的 20% III 型/80% I 型胶原蛋白凝胶的弹性比 100% I 型胶原蛋白（p < 0.05）更高，并且凝胶中的 HDF 具有较高的线粒体活性。因此，我们决定进一步评估 S-ESM 对无毛小鼠皮肤基因表达的影响，发现 S-ESM 显著增加了 III 型胶原蛋白。苦味酸红染色显示，S-ESM 处理后，乳头真皮中 III 型胶原蛋白明显增加。此外，S-ESM 的应用显著提高了人体手臂的弹性并减少了面部皱纹。ESM 可能通过增加 III 型胶原蛋白来减少组织弹性的丧失，从而延长寿命。
