Ebrahimian Neda, Montazeri Fatemeh, Sadeghi Mohammad Reza, Kalantar Seyed Mehdi, Gilany Kambiz, Khalili Mohannad Ali
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Clin Exp Reprod Med. 2020 Dec;47(4):293-299. doi: 10.5653/cerm.2019.03426. Epub 2020 Nov 19.
The goal of the present study was to investigate the rate of chromosomal aneuploidies in surplus embryos after sex determination at the cleavage stage. Then, the same chromosomal aneuploidies were evaluated in blastocysts after extended culture.
Sixty-eight surplus embryos were biopsied at the cleavage stage and incubated for an additional 3 days to allow them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 22, X, and Y) in both cleavage-stage embryos and blastocysts.
Although the total abnormality rate was lower in blastocysts (32.35%) than in cleavage-stage embryos (45.58%), the difference was not significant (p=0.113). However, when we restricted the analysis to autosomal abnormalities, we observed a significant difference in the abnormality rate between the cleavage-stage embryos (44.11%) and the blastocysts (17.64%, p=0.008). A higher rate of sex chromosomal abnormalities was also observed in cleavage-stage embryos (29.4%) than in blastocysts (14.70%, p=0.038).
The data indicated that embryo biopsy should be conducted at the blastocyst stage rather than the cleavage stage. The results also emphasized that examination of common chromosomal aneuploidies apart from sex selection cycles can be conducted in the blastocyst stage with the FISH method.
本研究的目的是调查卵裂期性别鉴定后多余胚胎的染色体非整倍体率。然后，在延长培养后的囊胚中评估相同的染色体非整倍体情况。
68个多余胚胎在卵裂期进行活检，并再培养3天使其发育至囊胚期。通过荧光原位杂交（FISH）对卵裂期胚胎和囊胚中的8条染色体（13、15、16、18、21、22、X和Y）进行重新分析。
尽管囊胚的总异常率（32.35%）低于卵裂期胚胎（45.58%），但差异不显著（p=0.113）。然而，当我们将分析限制在常染色体异常时，我们观察到卵裂期胚胎（44.11%）和囊胚（17.64%，p=0.008）的异常率存在显著差异。卵裂期胚胎的性染色体异常率（29.4%）也高于囊胚（14.70%，p=0.038）。
数据表明胚胎活检应在囊胚期而非卵裂期进行。结果还强调，除性别选择周期外，可在囊胚期采用FISH方法检测常见染色体非整倍体情况。
