Parasitology Laboratory, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Parasitology Laboratory, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Acta Trop. 2021 Feb;214:105762. doi: 10.1016/j.actatropica.2020.105762. Epub 2020 Nov 21.
Balantioides coli is a known ciliated zoonotic protozoan that mainly causes diarrhea in humans and pigs. An efficient and reliable culture system for this parasite remains unavailable until now. In this study, a modified Dulbecco's modified eagle medium (DMEM) with pH 7.0-7.5, containing 5 mg/mL starch and 20% new calf serum, was optimized for propagation of B. coli at 28°C-32°C. At the growth-peaking stage, the average trophozoite density was up to 12,970 trophozoites per milliliter. A reproducible protocol for isolation and maintenance of this parasite was also developed based on the modified DMEM culture medium. Moreover, cloning results of B. colipopulations showed that 250 trophozoites in 3 mL modified DMEM medium were the minimal number of trophozoites that propagated to the growth-peaking stage, and finally obtained the individual population. However, less than 250 trophozoites failed to continuously grow in the modified DMEM culture medium under the optimal conditions for growth of B. coli. These data showed that the modified DMEM culture medium is an ideal and efficient medium for propagation and maintenance of B. coli in vitro and will help studies on its biology, genome, transcriptome, proteome, and drug screening.
结肠内巴通体是一种已知的纤毛性人畜共患原生动物，主要导致人类和猪腹泻。直到现在，仍然没有一种有效的、可靠的该寄生虫培养系统。在这项研究中，优化了改良的 Eagle 氏最低必需培养基（DMEM），pH 值为 7.0-7.5，含有 5mg/mL 淀粉和 20%的新生小牛血清，用于在 28°C-32°C 下繁殖 B. coli。在生长高峰期，平均滋养体密度达到每毫升 12970 个滋养体。还基于改良的 DMEM 培养基开发了一种可重复的分离和维持该寄生虫的方案。此外，B. colipopulations 的克隆结果表明，在 3mL 改良 DMEM 培养基中，250 个滋养体是在 B. coli 生长的最佳条件下繁殖到生长高峰期并最终获得单个群体的最小滋养体数量。然而，在改良的 DMEM 培养基中，少于 250 个滋养体不能在最佳条件下连续生长。这些数据表明，改良的 DMEM 培养基是体外繁殖和维持 B. coli 的理想和有效培养基，将有助于其生物学、基因组、转录组、蛋白质组和药物筛选的研究。
