National Magnetic Resonance Research Center (UMRAM), Bilkent University, Ankara, Turkey; Interdisciplinary Neuroscience Program, Aysel Sabuncu Brain Research Center, Bilkent University, Ankara, Turkey; Department of Molecular Biology and Genetics Zebrafish Facility, Bilkent University, Ankara, Turkey.
Interdisciplinary Neuroscience Program, Aysel Sabuncu Brain Research Center, Bilkent University, Ankara, Turkey; Department of Molecular Biology and Genetics Zebrafish Facility, Bilkent University, Ankara, Turkey; National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey.
Neurobiol Aging. 2021 Feb;98:21-32. doi: 10.1016/j.neurobiolaging.2020.10.018. Epub 2020 Oct 23.
Understanding the principles underlying age-related changes in motion perception is paramount for improving the quality of life and health of older adults. However, the mechanisms underlying age-related alterations in this aspect of vision, which is essential for survival in a dynamic world, still remain unclear. Using optomotor responses to drifting gratings, we investigated age-related changes in motion detection of adult zebrafish (wild-type/AB-strain and ache mutants with decreased levels of acetylcholinesterase). Our results pointed out negative optomotor responses that significantly depend on the spatial frequency and contrast level of stimulation, providing supporting evidence for the visual motion-driven aspect of this behavior mainly exhibited by adult zebrafish. Although there were no significant main effects of age and genotype, we found a significant three-way interaction between contrast level, age, and genotype. In the contrast domain, the changes in optomotor responses and thus in the detection of motion direction were age- and genotype-specific. Accordingly, these behavioral findings suggest a strong but complicated relationship between visual motion characteristics and the cholinergic system during neural aging.
了解运动感知随年龄变化的原理对于提高老年人的生活质量和健康至关重要。然而，对于这一对于在动态世界中生存至关重要的视觉方面的年龄相关性改变的机制仍不清楚。我们使用光动反应来研究成年斑马鱼（野生型/AB 品系和乙酰胆碱酯酶水平降低的 ache 突变体）的运动检测随年龄的变化。我们的结果指出了负的光动反应，这显著依赖于刺激的空间频率和对比度水平，为这种行为的视觉运动驱动方面提供了支持证据，主要由成年斑马鱼表现出来。尽管年龄和基因型没有显著的主效应，但我们发现对比度水平、年龄和基因型之间存在显著的三向相互作用。在对比度域中，光动反应的变化以及运动方向的检测都具有年龄和基因型特异性。因此，这些行为学发现表明，在神经老化过程中，视觉运动特征与胆碱能系统之间存在着强烈但复杂的关系。
