Yang Fan, Yang Fang, Tu Ting-Ting, Liao Ni, Chai Ya-Qin, Yuan Ruo, Zhuo Ying
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China; College of Biological and Chemical Engineering, Panzhihua University, Panzhihua, 617000, PR China.
Biosens Bioelectron. 2021 Feb 1;173:112820. doi: 10.1016/j.bios.2020.112820. Epub 2020 Nov 16.
Developing low-cost and efficient methods to enhance the electrochemiluminescence (ECL) intensity of luminophores is highly desirable and challenging. Herein, we develop a synergistic promotion strategy based on three types of co-reaction accelerators to achieve an efficient SnO quantum dots (SnO QDs)-based ternary ECL system. Specifically, the MnO nanoflowers (MnO NFs), Ag nanoparticles (Ag NPs) and hemin/G-quadruplex were rationally selected as co-reaction accelerators. Owing to the synergistic effect, the deft integration of three types of co-reaction accelerators enabled better structural stability, more exposed catalytic active sites, and faster charge transfer, thus more effectively facilitating the reduction of co-reactant (SO) compared with that of the single co-reaction accelerator. To demonstrate the practical utility of this principle, an "on-off-super on" ECL biosensor was constructed in combination with a 3D DNA walker, which showed a superior linear range (10 aM-100 pM) and a low detection limit (2.9 aM) for the highly-sensitive miRNA-21 detection. In general, this work firstly reported that three types of co-reaction accelerators were deftly integrated to remarkably amplify the ECL emission of SnO QDs, and provided brand-new perspectives for research on the ingenious design of the structure and component of highly efficient co-reaction accelerators.
开发低成本且高效的方法来增强发光体的电化学发光（ECL）强度是非常必要且具有挑战性的。在此，我们基于三种共反应促进剂开发了一种协同促进策略，以实现基于高效氧化锡量子点（SnO QDs）的三元ECL体系。具体而言，合理选择了MnO纳米花（MnO NFs）、Ag纳米颗粒（Ag NPs）和血红素/G-四链体作为共反应促进剂。由于协同效应，三种共反应促进剂的巧妙整合实现了更好的结构稳定性、更多暴露的催化活性位点以及更快的电荷转移，因此与单一共反应促进剂相比，能更有效地促进共反应物（SO）的还原。为了证明这一原理的实际应用，结合3D DNA步行器构建了一种“关-开-超开”ECL生物传感器，该传感器在高灵敏度检测miRNA-21时显示出优异的线性范围（10 aM-100 pM）和低检测限（2.9 aM）。总体而言，这项工作首次报道了三种共反应促进剂的巧妙整合显著放大了SnO QDs的ECL发射，并为高效共反应促进剂的结构和组分的巧妙设计研究提供了全新的视角。
