Fan Xiaowei, Zhang Guping, Li Xiaodi, Wang Yao, Wang Yi, Hao Shilei, Liu Defang
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, No. 174, Shapingba Main Street, Chongqing 400030, China.
Biosensors (Basel). 2024 Dec 2;14(12):586. doi: 10.3390/bios14120586.
In this study, we have proposed an electrochemiluminescence (ECL) signal amplification system which is based on two-dimensional (2D) flower-like CdS@Co/Mo-MOF composites as a co-reaction accelerator of the g-CN/SO system for ultrasensitive detection of chlorpromazine hydrochloride (CPH). Specifically, the 2D flower-like Co/Mo-MOF with mesoporous alleviated the aggregation of CdS NPs while simultaneously fostering reactant-active site contact and improving the reactant-product transport rate. This allowed the material to act as a novel co-reaction accelerator, speeding up the transformation of the SO into SO and enhancing the cathodic ECL emission of g-CN. Moreover, the signal probe which was synthesized by coupling the 2D CdS@Co/Mo-MOF and graphitic carbon nitride (g-CN) achieved the generation of SO in situ and reduced energy loss. The results confirmed that the ECL signal was enhanced 6.2-fold and stabilized by CdS@Co/Mo-MOF. Based on the extremely strong quenching effect of chlorpromazine hydrochloride (CPH) on this system, a "signal-off" type sensor was constructed. The sensor demonstrated excellent sensitivity and linear response to CPH concentrations ranging from 1 pmol L to 100 μmol L, with a low detection limit of 0.4 pmol L (S/N = 3).
在本研究中，我们提出了一种基于二维（2D）花状CdS@Co/Mo-MOF复合材料的电化学发光（ECL）信号放大系统，该复合材料作为g-CN/SO系统的共反应促进剂用于超灵敏检测盐酸氯丙嗪（CPH）。具体而言，具有介孔的二维花状Co/Mo-MOF减轻了CdS纳米颗粒的聚集，同时促进了反应物-活性位点的接触并提高了反应物-产物的传输速率。这使得该材料能够作为一种新型的共反应促进剂，加速SO向SO的转化并增强g-CN的阴极ECL发射。此外，通过将二维CdS@Co/Mo-MOF与石墨相氮化碳（g-CN）偶联合成的信号探针实现了SO的原位生成并减少了能量损失。结果证实，CdS@Co/Mo-MOF使ECL信号增强了6.2倍并使其稳定。基于盐酸氯丙嗪（CPH）对该系统极强的猝灭作用，构建了一种“信号关闭”型传感器。该传感器对浓度范围为1 pmol L至100 μmol L的CPH表现出优异的灵敏度和线性响应，检测限低至0.4 pmol L（S/N = 3）。
