Wetter O, Brandhorst D, Breipohl W
Innere Universitätsklinik (Tumorforschung), West German Tumor Center, Essen, F.R.G.
Eur J Cancer Clin Oncol. 1987 Nov;23(11):1633-43. doi: 10.1016/0277-5379(87)90442-1.
An anti-idiotype antibody (anti-Id Ab) was made by immunization of a rabbit with the IgG-2/lambda paraprotein from a plasmacytoma patient (Sa). The antibody was directed against determinants of heavy (H) and light (L) chains on the F(ab)2 piece of the paraprotein. Using this antibody an unusual pattern of cytoplasmic fluorescence was seen with bone marrow cells of the patient. One population (type I) showed reactivity in association with gamma and lambda chains, the other (type II) with the anti-Id Ab only. So, by immunological methods, biclonality could be demonstrated using the anti-Id Ab but not with anti-isotype antibodies. L chains of mol. wt 23.5 and 17.0 kd were observed. The H and L chains of small molecular weight were not detected in the patient's serum nor in the culture supernatant. Thus, deleted and intracellularly degraded H and L chains may cause the unusual staining pattern of the type II cells.
通过用来自浆细胞瘤患者（Sa）的IgG - 2/λ副蛋白免疫兔子制备了一种抗独特型抗体（抗Id抗体）。该抗体针对副蛋白F(ab)2片段上重链（H）和轻链（L）的决定簇。使用这种抗体，在该患者的骨髓细胞中观察到了一种不寻常的细胞质荧光模式。一群细胞（I型）显示出与γ链和λ链相关的反应性，另一群细胞（II型）仅与抗Id抗体反应。因此，通过免疫学方法，使用抗Id抗体可以证明双克隆性，而使用抗同种型抗体则不能。观察到分子量为23.5和17.0kd的轻链。在患者血清和培养上清液中均未检测到小分子的重链和轻链。因此，缺失的和细胞内降解的重链和轻链可能导致II型细胞出现不寻常的染色模式。
