Renau-Piqueras J, Wetter O, Miragall F, Brandhorst D, Martinez-Ramon A
Leuk Res. 1984;8(3):491-500. doi: 10.1016/0145-2126(84)90090-0.
Mononuclear cells from a bone marrow infiltrated by plasmacytoma cells were examined by electron microscopy. An analysis was performed according to different cytoarchitectural forms of the endoplasmic reticulum (ER). Six types of plasma cells could be distinguished. The bone marrow cells were treated with an anti-idiotype antiserum from a guinea pig prepared against the patient's monoclonal serum protein and with a FITC conjugated anti-guinea pig antiserum from the rabbit as second layer. Then the cells were passed through an affinity gel column with anti-FITC antibodies. The original preparation and the cells separated on the affinity gel were analysed by electron microscopy. It was found that an ultrastructurally distinct type of plasma cell was enriched 3.5-fold over the original sample by the separation procedure.
对被浆细胞瘤细胞浸润的骨髓中的单核细胞进行了电子显微镜检查。根据内质网（ER）不同的细胞结构形式进行了分析。可区分出六种类型的浆细胞。用针对患者单克隆血清蛋白制备的豚鼠抗独特型抗血清以及作为第二层的兔抗豚鼠异硫氰酸荧光素（FITC）偶联抗血清处理骨髓细胞。然后将细胞通过带有抗FITC抗体的亲和凝胶柱。通过电子显微镜对原始制剂和在亲和凝胶上分离的细胞进行分析。结果发现，通过分离程序，一种超微结构上独特类型的浆细胞比原始样品富集了3.5倍。
