Restorative Dentistry Department, Dental Materials Division, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil; Cariology and Operative Dentistry Department, Tokyo Medical and Dental University, Tokyo, Japan; General Dentistry Department, Division of Biomedical Materials, East Carolina University School of Dental Medicine, Greenville, NC, USA.
Restorative Dentistry Department, Dental Materials Division, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil.
Dent Mater. 2021 Jan;37(1):120-130. doi: 10.1016/j.dental.2020.10.026. Epub 2020 Nov 21.
To a self-etch adhesive doped with nano-bioglass and evaluate its ability to bond and re-mineralize artificially demineralized dentin.
Experimental Si, Ca, Na and PO based nanobioglass particles were synthesized, doped into experimental self-etch adhesives, and divided into 3 groups: Clearfi SE2 (CSE2), experimental (EXC), and experimental doped with 10% of nanobioglass (ExNB). The adhesives were applied onto the caries-affected dentin (chemically simulated), and evaluated after 24 h and 28 days of immersion in simulated body fluid. The remineralization process was assessed using optical coherence tomography, nanoindentation, in situ zymography, transmission electron microscopy, confocal laser scanning microscopy, μ-tensile bond strength, and pH buffer.
The addition of nanobioglass particles into the experimental self-etch adhesives altered the μTBS in the short-term jeopardizing dentin bonding properties, when compared to the non-doped self-etch adhesive. The remineralization recovered the nanohardness, and volume lost by caries lesion (p = 0.02). Moreover, reduced the enzymatic activity (p = 1.24) and formed new crystals within of the hybrid layer.
The use of nanobioglass was efficient to recover the properties of a caries affected dentin. Furthermore, the adhesive properties were not hampered and the probabilistic reliability increased.
研究一种自酸蚀黏合剂中掺杂纳米生物玻璃的能力，评估其对人工脱矿牙本质的黏结和再矿化能力。
合成基于 Si、Ca、Na 和 PO 的实验性纳米生物玻璃粒子，将其掺杂到实验性自酸蚀黏合剂中，并分为 3 组：Clearfi SE2（CSE2）、实验组（EXC）和实验组中掺杂 10%纳米生物玻璃的组（ExNB）。将黏合剂应用于龋损牙本质（化学模拟），并在模拟体液中浸泡 24 小时和 28 天后进行评估。使用光相干断层扫描、纳米压痕、原位酶谱、透射电子显微镜、共聚焦激光扫描显微镜、μ-拉伸黏结强度和 pH 缓冲液评估再矿化过程。
与未掺杂自酸蚀黏合剂相比，纳米生物玻璃颗粒的添加在短期内改变了μTBS，从而影响了牙本质的黏结性能。再矿化恢复了纳米硬度和龋损引起的体积损失（p = 0.02）。此外，还降低了酶活性（p = 1.24）并在混合层内形成了新的晶体。
纳米生物玻璃的使用有效地恢复了受龋影响的牙本质的性能。此外，黏结性能不受影响，概率可靠性增加。
